卵巢早衰患者外周血CD4^+CD25^+Treg细胞的变化及意义  

Changes and Significances of CD4^+CD25^+T Regulatory Cells in Patients with Premature Ovary Failure

作　　者：谢江燕[1] 东亚君[1] 梁志清[1] 何畏[1] 

机构地区：[1]第三军医大学西南医院妇产科,重庆400038

出　　处：《生殖与避孕》2013年第4期224-227,共4页Reproduction and Contraception

基　　金：国家自然科学基金项目;项目号:30973198

摘　　要：目的:探讨CD4+CD25+调节性T细胞(即CD4+CD25+Treg细胞)在卵巢早衰发病机制中的作用。方法:流式细胞仪定量检测卵巢早衰(premature ovarian failure,POF)患者、卵巢储备功能下降(diminished ovarian reserve,DOR)患者及健康对照组外周血CD4+T、CD8+T细胞及CD4+CD25+Treg细胞数量,应用3H-thymidine掺入法测定POF患者及对照组外周血CD4+CD25+Treg细胞对效应性T细胞的增殖抑制功能。结果:与对照组相比,POF患者及DOR患者CD4+CD25+Treg细胞比例降低(P<0.01)、POF患者CD4+T/CD8+T细胞比值增高(P<0.05),DOR患者CD4+T/CD8+T细胞比值无明显变化(P>0.05);POF患者免疫抑制功能无明显降低(P>0.05)。结论:CD4+CD25+Treg细胞比例降低与T细胞亚群失衡可能是POF的发病机制。Objective： To explore the role of T lymphocyte subsets and CD4＋CD25＋T regulatory cells （CD4＋CD25＋Treg cells） in the pathogenesis of premature ovary failure （POF）. Methods： Flow-cytometry analysis of CD4＋T, CDS＋T cells and CD4＋CD25＋Treg cells was performed on fresh peripheral blood in patients with POF （n=26）, diminished ovarian reserve （DOR, n=19） and control group, the suppression function of CD4＋CD25＋Treg cells in POF group and the control were detected by 3H-thymidine experiment. Results： Compared with the control, the percentages of CD4＋CD25＋Treg cells in POF group and DOR gruop were obviously reduced （P〈0.01）, and the CD4＋T/CDS＋T ratio in POF group was significantly in- creased （P〈0.05）; but in DOR group, there was no significant difference; however the suppression func- tion of CD4＋CD25＋Treg cells in POF group was not significantly decreased （P〉0.05）. Conclusion： The decreased CD4＋CD25＋Treg ceils and the imbalance of T lymphocyte subsets may play an important role in the pathogenesis of POF.

关 键 词：卵巢早衰(POF) 卵巢储备功能下降 CD4+CD25+调节性T细胞(CD4+CD25+Treg细胞) T淋巴细胞亚群 

分 类 号：R711.75[医药卫生—妇产科学]