清热消积方对人肺腺癌细胞诱导的人脐静脉内皮细胞迁移、趋化和成管能力的影响  

Effects of Qingre Xiaoji Recipe on the Migration, Chemotaxis, and Tube Formation Capability of Human Lung Adenocarcinoma Cell Induced Human Umbilical Vein Endothelial Cells

作　　者：陈培丰[1] 潘磊[1] 金莹祺[2] 

机构地区：[1]浙江中医药大学附属第一医院肿瘤科,杭州310006 [2]浙江中医药大学附属第二医院康复科,杭州310007

出　　处：《中国中西医结合杂志》2013年第4期497-501,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省教育厅重点资助项目(No.Z2009-07128)

摘　　要：目的研究清热消积方对人肺腺癌细胞(SPC-A-1)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)迁移、趋化和成管能力的影响。方法设终浓度分别为2.5、5.0、10.0、20.0mg/mL的清热消积方给药组和对照组,运用划痕、Transwell小室、Matrigel胶小管形成等实验方法观察细胞迁移趋化效应及成管能力。结果 2.5、5.0、10.0mg/mL的清热消积方药液对由SPC-A-1细胞上清培养的HUVEC细胞的迁移运动均有不同程度的抑制作用(P<0.05),且迁移率随着药物浓度的增加而下降;HU-VEC细胞的趋化细胞数明显少于阳性对照组(P<0.01),趋化抑制率随着药物浓度的增加而上升,且HU-VEC细胞趋化数目与药物浓度呈负相关(r=-0.830,P<0.01);HUVEC细胞小管形成面积明显低于阳性对照组(P<0.01),小管形成抑制率随着药物浓度的增加而上升,且HUVEC细胞生成小管的面积与药物浓度呈负相关(r=-0.937,P<0.01)。结论清热消积方抗肿瘤血管生成作用可能与其抑制肿瘤血管内皮细胞的迁移、趋化及成管能力等相关。Objective To study the effects of Qingre Xiaoji Recipe （QXR） on the migration, chemotaxis, and tube formation capability of human umbilical vein endothelial cells （HUVECs） induced by human lung adenocarcinoma cells （SPC-A-1）. Methods The QXR groups at different final concentrations （2.5, 5.0, 10.0, and 20.0 mg/mL） and a control group were set up. Then the chemotaxis and tube formation oapabilities were observed using Erasion trace test, Transwell chamber assay, and Matrigel matrix tube formation assay. Results QXR at three concentrations （2.5, 5.0, and 10.0 mg/mL） had inhibition on the migration of HUVECs cultured by SPC-A-1 cell supernatant （P0.05）. Besides, the migration rate decreased along with increased concentrations. The number of chemotactic HUVECs was significantly less than that of the positive control group （P0.01）, and the inhibition rate of chemokine increased along with increased concentrations. Furthermore, the number of chemotactic HUVECs was negatively correlated to the drug concentration （r=-0.830, P0.01）. The area of HUVEC cell tubule formation was significantly lower than that of the positive control group （P0.01）. The inhibition rate of HUVEC cell tubule formation increased along with increased drug concentration. Besides, the area of HUVEC cell tubular formation was negatively correlated with the drug concentration （r=-0.937, P0.01）. Conclusion The mechanism of QXR for fighting against tumor angiogenesis might be correlated with its inhibition on the migration, chemotaxis, and tube formation of tumor vascular endothelial cells.

关 键 词：清热消积方 人肺腺癌细胞株 人脐静脉内皮细胞 抗肿瘤血管生成 

分 类 号：R285.5[医药卫生—中药学]