含铜不锈钢的体外抗菌及细胞相容性研究  

In Vitro Evaluation of Antibacterial Activity and Cytocompatibility of Antibacterial Stainless Steel Containing Copper

作　　者：管俊林[1] 郭磊[1] 付玉平[1] 柴宏伟[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001

出　　处：《生物医学工程学杂志》2013年第2期333-337,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30872724;81071460);辽宁省教育厅科研项目资助(L20107125;L2010711);辽宁省科技攻关层次计划项目资助(2008225009;2009225010-3)

摘　　要：为明确含铜4.5%的317L不锈钢的体外抗菌性能及其生物相容性,通过制备分布着4.5%的ε-Cu相的317L不锈钢(317L-Cu),应用体外细胞直接接触实验检测317L-Cu材料表面成骨细胞的生长形态及增殖,并评价体外317L-Cu材料的抗菌性能。结果表明,317L-Cu材料在体外具有很好的抗菌性能,可以有效杀灭材料表面大肠杆菌;317L-Cu材料与成骨细胞直接接触培养48h,材料表面的成骨细胞生长良好,细胞增殖活性与钛合金材料比较无显著性差异。因此,317L-Cu具有良好的细胞相容性,有望成为具有抗菌性新型医用生物材料。To study the in vitro antibacterial activity and biocompatibility of 317L stainless steel containing 4.5% copper alloy （317L-Cu）, we produced 317L-Cu stainless steel withe-Cu phase. The cell proliferation of osteoblasts on material surface was detected in vitro. Escherichia coli was cultured with 317L-Cu for evaluating the antibacterial activity. We found that the 3ITL-Cu could effectively kill the Escheriehia coli on material surface. The cell prolifera- tion of osteoblasts on material surface was not different significantly, compared with titanium material. Our study clearly demonstrated that the 317L-Cu alloys had a significant antimicrobial activity and was biocompatible in vitro, so it would be suitable to be used as a new medical material with antibacterial activity.

关 键 词：不锈钢 铜 抗菌性 细胞增殖 

分 类 号：R318.08[医药卫生—生物医学工程]