片状材料对血小板激活作用的体外评价  

In vitro Evaluation of the Function of Sheet Biomaterials in Platelet Activation

作　　者：许建霞[1] 王志杰[2] 王春仁[1] 奚廷斐[3] 

机构地区：[1]中国食品药品检定研究院医疗器械检验中心,北京100050 [2]华南理工大学生物医学工程研究院,广州510640 [3]北京大学前沿交叉学科研究院生物医用材料与组织工程研究中心,北京100871

出　　处：《生物医学工程学杂志》2013年第2期338-341,共4页Journal of Biomedical Engineering

基　　金：科技部"863"课题资助项目(2011AA020104)

摘　　要：对生物材料血液相容性的评价,通常局限于溶血、凝血时间、血小板黏附等实验。本研究旨在建立一种评价片状材料对血小板激活作用的方法。本研究采用一种合理的血液与片状材料接触模型,以血浆血小板α-颗粒膜蛋白(GMP-140)为检测指标,对玻璃、聚氯乙烯、聚甲基乙烯基硅氧烷3种基础材料片在不同震荡速率下对血小板的激活作用进行了比较。结果显示:在140r/m和200r/m的震荡速率下,3种材料对血小板激活作用的差异不能完全区分开;而在230r/m的震荡速率下,3种材料对血小板的激活作用的差异具有统计学意义,玻璃组>聚氯乙烯组>硅橡胶组。但在270r/m的震荡速率下,顺序完全相反,可能是受到界面张力的影响。本研究表明该方法适用于片状材料对血小板激活作用的评价,但应注意选择合适的震荡速率;同时,界面张力在该模型中起重要作用,结果评价时须考虑其影响。In China, the evaluation of hemoeompatibility of biomaterials is limited to hemolysis, coagulation time,and the number and morphology of platelets adhered on biomaterials. The prdsent research,however, is aimed to estab- lish a method for evaluating the function of sheet biomaterials in platelet activation. Platelet activation caused by glass, polyvinyl chloride or polymethylvinylsiloxane sheets was evaluated by measuring α-granule membrane protein （GMP-140） in platelet poor plasma, using a reasonable blood-material contact model vibrating at different speed. The result showed that the difference in platelet activation was not significantly different among the three above-men- tioned materials at 140r/m or 200r/m. However, when it comes to 230r/m, significant difference was observed a- mong these three groups, with glass〉 polyvinyl chloride〉 polymethylvinylsiloxane. But the order was reversed at 270r/m, which may be due to the different interfacial tension of different materials. Therefore, the method is suit- able to evaluate platelet activation caused by sheet biomaterials, but an appropriate vibrating speed should be chosen. The interracial tension plays an important role in the model and should be considered for results assessment.

关 键 词：片状材料 血小板激活 Α-颗粒膜蛋白 

分 类 号：R318.08[医药卫生—生物医学工程]