人参茎叶皂苷对D-半乳糖大鼠不同部位骨骼的影响  

Effect of ginsenoside from the stem and leaf on different skeletal sites of osteoporotic rats induced by D-galactose

作　　者：张新乐[1] 吴铁[1] 崔燎[1] 刘钰瑜[1] 姚卫民[1] 许碧莲[1] 邹丽宜[1] 陈文双[1] 

机构地区：[1]广东医学院药理教研室,湛江524023

出　　处：《中国骨质疏松杂志》2013年第3期251-257,共7页Chinese Journal of Osteoporosis

基　　金：广东省教育厅育苗工程项目(08-NYM-061)

摘　　要：目的建立D-半乳糖大鼠骨质疏松模型,通过骨形态计量学观察人参茎叶皂苷对大鼠不同部位的影响。方法选取3月龄雄性SD大鼠50只,随机分为正常对照组(NS)、D-半乳糖模型组(DG)、复方司坦唑醇阳性对照组(CP)、人参茎叶皂苷低剂量组(GSLL)、人参茎叶皂苷高剂量组(GSLH),每组10只。给药14周后处死大鼠,取胫骨上段、胫骨中段,进行骨组织形态计量学参数检测;取左侧股骨检测骨钙、磷以及骨胶原的含量。结果高剂量的人参茎叶皂苷可使D-半乳糖大鼠胫骨上段松质骨的骨小梁面积百分数(%Tb.Ar)增加、骨小梁数目(Tb.N)明显增加,而骨小梁分离度(Tb.Sp)明显减少,动态参数无明显变化,骨吸收参数明显减少;使D-半乳糖大鼠胫骨中段的皮质骨面积百分数(%Ct.Ar)增加,骨髓腔面积百分数(%Ma.Ar)减少,骨内外膜的形成和矿化均无明显变化;高低剂量组的股骨干湿重体重比无明显变化,低剂量的人参茎叶皂苷的骨羟脯氨酸增加。结论人参茎叶皂苷能有效对抗D-半乳糖大鼠胫骨上段和中段骨的丢失,高剂量效果好;低剂量的人参茎叶皂苷可以提高D-半乳糖大鼠骨有机质的相对含量。Objective To establish the model of osteoporotic rats induced by D-galactose, and to investigate the effect of ginsenoside from the stem and leaf on different skeletal sites of osteoporotic rats using bone histomorphometry. Methods Fifty 3-month-old male Sprague-Dawley （ SD ） rats were randomly divided into normal control group, D-galactose group, CP group, and ginsenoside from the stem and leaf at low dose （GSLL） or high dose group （GSLH）. Rats were sacrificed in 14 weeks after drug administration. The longitudinal proximal tibial metaphyseal （PTM） and tibial shaft （Tx） sections were used for bone histomorphometric analysis. The left femur was used for testing bone calcium （Ca） , phosphate （P） , and bone hydroxyproline. Results Comparing to those in D-galactose group, % Tb. Ar and Tb. N in PTM of rats in GSLH group were increased, while Tb. Sp was decreased. No changes of dynamic indexes were observed. Bone resorption decreased significantly. % Ct. Ar in Tx was increased, while % Ma. Ar was decreased. No changes occurred in the formation of internal and external bone membrane and mineralization. There was no change of wet and dry weight of Tx between GSLL and GSLH group. Hydroxyproline content in GSLL group was significantly increased. Conclusion GSL can effectively resist bone loss in PTM and Tx in osteoporotic rats induced by G-galactose. High dose of GSL has better efficacy. Low dose of GSL can increase the relevant amount of organic materials in osteoporotic rats induced by D-galaetose.

关 键 词：人参茎叶皂苷 D-半乳糖 骨质疏松 骨组织形态计量学 

分 类 号：R285[医药卫生—中药学]