耐高温小球藻紫外诱变育种及其耐高温性质研究  

UV mutagenesis breeding for high temperature resisting Chlorella and its properties of thermotolerance

作　　者：夏金兰[1,2] 宁进军[1] 陈程浩[1] 万民熙[1] 聂珍媛[1,2] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083 [2]中南大学生物冶金教育部重点实验室,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2013年第3期867-873,共7页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(50621063);湖南省研究生创新学位论文资助项目(2010ssxt246);中南大学创新教育资助项目(2010bsxt05;CL11029;CL11038)

摘　　要：从内蒙古筛选得到1株淡水小球藻Chlorella sorokiniana CS-01,采用原生质体-紫外线诱变技术,以55℃高温培养为选择压力,得到1株能在该温度下存活的突变藻株,该突变藻株能够适应利用烟道气CO2培养造成的高温环境。突变藻株适应的温度范围更广（15~55℃）,生长周期缩短,生物质产量增加;当通入装机容量为500MW的火力发电厂烟道气,其生长周期较Chlorella sorokiniana CS-01缩短2d,油脂质量分数提高20%。传代培养5代,生长情形相同,具有较好的遗传稳定性;该藻株具有火电厂、冶金厂、水泥厂等高排放工业位点固定烟道气CO2的应用价值,生物质经油脂提取和转酯反应可用于制取生物柴油。A fresh water microalga named Chlorella sorokiniana CS-01 which was isolated from Inner Mongol was mutated by UV mutagenesis of protoplasts and screened at high culture temperature（55℃）,the mutated microalga can withstand high temperature caused by the flue gas.It has a wider growth temperature（15-55℃）and shorter life cycle, and a higher biomass yield.Pumping into a simulative 500 MW thermal power plant flue gas at 38℃,its life cycle is shortened by 2 d and the oil content was raised by 20%.This mutated microalga has good stability of heredity,and it has considerable commercial value for fixing CO2 of flue gases from high CO2 emission sites,e.g.thermo-power plants, metallurgical plants and cement plants.The biomass by oil-extraction and esterification can be used to produce biodiesel.

关 键 词：紫外诱变 小球藻 耐高温性 生物柴油 烟道气 

分 类 号：Q939.97[生物学—微生物学]