伊曲康唑联合卡泊芬净或特比萘芬对球形孢子丝菌体外抑菌作用的研究  

In vitro interactions of itraconazole with caspofungin or terbinafine against Sporothrix globosa

作　　者：谭静文[1,2] 刘伟[1,2] 刘伟霞[1,2] 张晋卿[1,2] 孙毅[1,2] 万喆[1,2] 李若瑜[1,2] 

机构地区：[1]北京大学第一医院皮肤性病科 [2]北京大学真菌和真菌病研究中心皮肤病分子诊断北京市重点实验室,北京100034

出　　处：《中国真菌学杂志》2013年第1期20-25,共6页Chinese Journal of Mycology

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0198);国家自然科学基金重点项目(30930006);国家自然科学基金(30970131);北京市自然科学基金(7102149)

摘　　要：目的探讨伊曲康唑联合卡泊芬净或特比萘芬时对球形孢子丝菌酵母相和菌丝相的体外抑菌作用。方法参照标准的微量液基稀释法及棋盘微量稀释法对33株球形孢子丝菌行药敏试验,结果使用抑菌浓度指数(FICI)判定"协同""不相关"和"拮抗"。结果伊曲康唑与卡泊芬净联合对球形孢子丝菌酵母相菌株的协同率为93.94%(31/33),与特比萘芬联合的协同率为60.61%(20/33);伊曲康唑与卡泊芬净或特比萘芬联合对菌丝相菌株协同率分别为96.97%(32/33)、84.5%(28/33)。对所有受试菌株均未观察到上述药物的拮抗作用。结论体外联合药敏试验结果显示伊曲康唑与卡泊芬净或特比萘芬联合有较好的协同作用。Sporotrichosis is a common fungal infection with limited therapeutic strategies. Combination of synergistic drugs could provide new angle for treating this disease. We used the standard broth microdilution and checkerboard method to evaluate the in vitro efficacy of combining itraconazole with caspofungin or terbinafine against the yeast and mycelial phases of Sporothrix globosa. Fractional inhibitory concentration index （FICI） was used to interprete the result as following： synergistic if the FICI is ≤0.5; in- different if the FICI is 〉0.5 but ≤4 and antagonistic if the FICI is 〉4. For yeast phase, the synergic interaction of itraconazole combined with caspofungin or terbinafine was 93.94% （31/33） and 60.61% （20/33） , respectively; for mycelial phase, the syn- ergic interaction of itraconazole combined with caspofungin or terbinafine was 96.97 % （ 32/33 ） and 84.5 % （20/33） , respectively. Antagonism was not observed. The in vitro efficacy of combining itraconazole with caspofungin or terbinafine showed perfect synergy against S. globosa , suggesting a potential therapeutic choice for treatment of Sporotrichosis.

关 键 词：球形孢子丝菌 菌丝相 酵母相 卡泊芬净 伊曲康唑 特比萘芬 联合药敏 

分 类 号：R978.5[医药卫生—药品]