苦瓜枯萎病抗性鉴定与抗性遗传规律研究  

Studies on the Identification Technology and Inheritance of Disease Resistance to Fusarium Wilt in Bitter Gourd

作　　者：赵秀娟[1,2] 唐鑫[2] 胡开林[2] 

机构地区：[1]广东科贸职业学院,广州510430 [2]华南农业大学园艺学院,广州510642

出　　处：《园艺学报》2013年第4期685-692,共8页Acta Horticulturae Sinica

基　　金：广东省教育部产学研结合项目(2012B091100167);广东省科技计划项目(20120201);广东现代农业产业技术体系建设专项资金项目

摘　　要：以4份田间抗性水平不同的苦瓜为材料,探讨了适用于苦瓜苗期人工接种枯萎病菌的抗性鉴定方法;以此为基础,对来自国内外的43份苦瓜种质资源进行了抗源筛选,以其中的抗病亲本‘0417’和感病亲本‘472113’为材料,研究了苦瓜对枯萎病抗性的遗传规律。结果表明,直接水培接种法是较适合于苦瓜苗期枯萎病抗性鉴定的方法,适宜的接种菌液孢子浓度为4×106.mL-1。在苦瓜种质资源中,枯萎病抗源普遍存在,尤以野生种或半栽培种抗病性较强。苦瓜枯萎病抗性受单一显性核基因控制,其广义遗传力为90.78%。The identification technology of resistance to Fusarium wilt in Momordica charantia was investigated with inbred lines presenting different degree of field resistance. Then a total of 43 Momordica charantia varieties （or inbred lines） from home and abroad were evaluated for resistance to Fusarium wilt. Furthermore, a highly susceptible inbred line ＇472113＇ and a highly resistant inbred line ＇0417＇ were used to study the inheritance of resistance to Fusarium wilt in Momordica charantia. The results indicated that direct hydroponic inoculation with concentration of 4 x 106 spores per milliliter was most suitable for resistance identification. And the existence of resistant resource is prevalent among the Momordica charantia germplasm resources. The broad-sense heritability of Momordica charantia resistance to Fusarium wilt is 90.78%. The resistance is controlled by single dominant nuclear gene without affecting by cytoplasm.

关 键 词：苦瓜 枯萎病 苗期接种 抗性遗传 

分 类 号：S642.5[农业科学—蔬菜学]