梨两个休眠相关MADS-box基因特征及在其休眠过程中的表达分析  

Characteristics of Two Dormancy-associated MADS-box Genes in Pear and Their Expression Analysis During the Dormancy

作　　者：刘国琴[1,2] 郑鹏华[1] Sayed Hussain 滕元文[1] 

机构地区：[1]浙江大学园艺系,农业部园艺植物生长发育与品质调控重点开放实验室,杭州310058 [2]贵州大学农学院,贵阳550025

出　　处：《园艺学报》2013年第4期724-732,共9页Acta Horticulturae Sinica

基　　金：国家现代梨产业技术体系建设专项项目(nycytx-29-14)

摘　　要：PpMADS1和PpMADS2是从‘酥梨’(Pyrus pyrifolia white pear group‘Suli’)休眠芽转录组文库筛选的两个与休眠相关的MADS-box基因序列。为了解序列的特征,对其进行了相关生物信息学分析,并以‘翠冠’和‘圆黄’梨为试材,用实时定量PCR技术分析其花芽休眠不同阶段的表达变化。结果表明:两个基因都具有MADS-box家族的特征序列MIKC-基序,PpMADS1和PpMADS2分别与日本梨(P.pyrifolia‘Kosui’)休眠相关的两个MADS-box基因PpMADS13-1和PpMADS13-2聚在一起,且单独聚为一支,与李属植物休眠相关的MADS-box基因关系最近。在两个品种的休眠过程中,PpMADS1和PpMADS2的表达呈现相似的变化趋势,都有一个表达高峰,但‘翠冠’梨PpMADS1和PpMADS2的表达高峰均出现在11月15日,而‘圆黄’梨PpMADS1的表达高峰延后到12月30日,PpMADS2的表达高峰出现在12月15日。两个品种PpMADS1和PpMADS2的表达高峰都出现在内休眠解除之前,随内休眠解除其表达量下调,在生态休眠阶段表达量维持在较低的水平。据此推测PpMADS1和PpMADS2的表达对梨芽内休眠的解除具有调控作用。Two dormancy-associated MADS-box genes （PpMADS1 and PpMADS2） were obtained from transcriptome library constructed Pyrus pyrifolia white pear group ＇ Suli＇ . To understand sequence characteristics of two genes, bioinformatics analysis was carried out. Furthermore the gene expression patterns in lateral flower buds of both pear cultivars ＇ Cuiguan＇ and ＇Wonhwang＇ were analyzed during the period of dormancy by quantitative real-time PCR. The results showed that PpMADS1 and PpMADS2 contained well-conserved MIKC-motifs of MADS-box. A phylogenetic analysis revealed that PpMADS1 was closely related to PpMADS13-1 of Japanese pear （P. pyrifolia ＇Kosui＇ ）, whereas PpMADS2 was similar to PpMADS13-2 of Japanese pear. The pear DAM genes formed an independent subclade and were closely related to those of Prunus spp. The expression patterns of PpMADS1 and PpMADS2 were similar with one peak in two cultivars during the period of dormancy. However, the timing of gene expression peak differed between two cultivars. The PpMADS1 and PpMADS2 expression peak occured on the November 15th in ＇Cuiguan＇ pear, while the PpMADS1 expression peak on the December 30th, and the PpMADS2 expression peak on the December 15th in ＇Wonhwang＇ pear. The PpMADS1 and PpMADS2 expression peak appeared prior to the release of endodormancy, and their expression levels were gradually down-regulated after the turn over of endodormancy and kept at a lower level during the period of ecodormancy. These results indicated that PpMADS1 and PpMADS2 might play an important role in the regulation of bud endodormancy-release in pear.

关 键 词：梨 芽 休眠 MADS-BOX基因 表达分析 

分 类 号：S661.2[农业科学—果树学]