外源性结缔组织生长因子在人毛囊混合细胞重建组织工程毛囊中的作用  

Functions of exogenous application of connective tissue growth factor in stimulating human dermal papilla cells and human hair follicle outer root sheath cells for reconstructive tissue-engineering hair follicles

作　　者：张培培[1] 柴家科[1] 王继萍[1] 段红杰[1] 马丽[1] 朱桂英[1] 

机构地区：[1]解放军总医院第一附属医院烧伤整形外科,北京100048

出　　处：《中华医学杂志》2013年第14期1063-1066,共4页National Medical Journal of China

摘　　要：目的 探讨外源性结缔组织生长因子（CTGF）在人毛囊混合细胞重建组织工程毛囊中的作用。方法采用细胞培养技术体外培养人毛囊外根鞘细胞及人毛乳头细胞，人毛囊外根鞘细胞与人毛乳头细胞以5：1的比例制备细胞混悬液。流式细胞学检测人毛囊外根鞘细胞中CD200阳性细胞含量。取8只裸鼠用随机数字表法均分为A、B组，制作裸鼠背部创面。A组移植添加20μg／LCTGF的细胞混悬液，B组移植单纯的细胞混悬液。于8周后进行组织学检查，观察毛囊形成情况。采用普通PCR检测移植物组织中人特异性DNA及鼠DNA表达情况。结果流式细胞学检测结果表明培养的人毛囊外根鞘细胞中CD200阳性细胞占细胞总量19．65％。组织学检查显示A组毛囊样结构形成数量（268±96）显著多于B组（62±20），差异有统计学意义（P〈0．05）。PCR结果表明移植物中含有人组织成分。结论CTGF可促进人毛囊外根鞘细胞与人毛乳头细胞相互作用，诱导毛囊样结构形成。Objective To explore the functions of connective tissue growth factor （CTGF） in the restoration of hair follicles with a mixture of human dermal papilla cells and human hair follicle outer root sheath cells in vitro in nude mice. Methods Human hair follicle outer root sheath ceils （hfORS） and human hair dermal papilla cells （hDP） were cultured in vitro and mixed in a fixed ratio （ hfORS ： hDP = 5 ： 1 ）. Flow cytometry was used to detect the content of CD200 ＋ cells in human hair follicle outer root sheath cells. And 8 nude mice were divided randomly into 2 groups according to a random number table and back wounds produced. Group A was transplanted with cell mixture plus 20 μg/L CTGF. Group B was transplanted with cell mixture alone. After 8 weeks of transplantation, the development of hair follicle formation was observed histologically. PCR was used to detect the expression of human specific DNA and mice DNA in transplants. Results The portion of CD200 cells in cultured hfORS was 19.65%. At 8 weeks after implantation, hair follicle formation could be observed in Group A （268 ± 96） more than Group B （ 62± 20 ）. The difference was statistically significant （ P 〈 0. 05 ）. And PCR showed that there was human composition in transplant. Conclusion CTGF can induce the formation of hair follicle by promoting the interference between hDP and hfORS.

关 键 词：结缔组织生长因子 毛囊 小鼠 裸 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]