Rationalizing the isolation distance needed for field trials involving genetically modified rapeseed (Brassica napus L.) in China  

作　　者：ZHAO XiangXiang TANG Tang CHEN GuiMin LIU FuXia WANG XingLong BU CuiPing LU ChangMing 

机构地区：[1]Key Laboratory of Oil Crop Biology of Ministry of Agriculture,Oilcrops Research Institute,Chinese Academy of Agricultural Sciences [2]Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake,Huaiyin Normal University

出　　处：《Chinese Science Bulletin》2013年第13期1558-1567,共10页

基　　金：supported by the National Natural Science Foundation of China (30900217);the Public Welfare Industry-Special Research Project of Ministry of Environmental Protection (201109028);Natural Science Foundation of Jiangsu Province (BK2011410);"333" High-level Personnel Training Project of Jiangsu Province;Jiangsu Province "Cyan-blue"Cultivation Project for Middle-aged academic leaders;"Cyan-blue"Project for Outstanding Scientific and Technological Innovation Team of Jiangsu Province

摘　　要：The isolation distance required for field trials of genetically modified (GM) rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow experiment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate (y) with distance (x) was well described by the equation y = 1.3936x-0.9136 (R 2 = 0.9950). The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow (less than 0.01%) under conditions of winter rapeseed production in China.The isolation distance required for field trials of genetically modified （GM） rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow ex- periment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate （y） with distance （x） was well described by the equation y = 1.3936x-0.9136 （R2 = 0.9950）. The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow （less than 0.01%） under conditions of winter rapeseed production in China.

关 键 词：转基因油菜籽 隔离距离 现场试验 中国 转基因植物 花粉流 世界范围 田间试验 

分 类 号：S565.4[农业科学—作物学] S641