脓毒症患者中血清程序化细胞死亡因子5水平的变化  

Serum level of programmed cell death 5 protein levels in patients with sepsis

作　　者：刘慧琳[1] 田勍[2] 洪天配[2] 刘桂花[1] 潘欢[3] 王海宁[2] 高洪伟[2] 

机构地区：[1]北京大学第三医院急诊科,北京100191 [2]北京大学第三医院内分泌科,北京100191 [3]北京大学人类疾病基因研究中心,北京100191

出　　处：《北京大学学报（医学版）》2013年第2期238-241,共4页Journal of Peking University:Health Sciences

摘　　要：目的:探讨血清程序化细胞死亡因子5(programmed cell death 5 protein,PDCD5)水平在全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)、脓毒症及重症脓毒症患者中的变化。方法:78例患者纳入本研究,其中SIRS(脓毒症除外)患者28例、脓毒症(重症脓毒症除外)患者23例、重症脓毒症患者27例,并选取健康对照22例,分别收集其静脉血清,采用酶联免疫吸附法检测PDCD5水平,比较PDCD5水平的差异。采用Spearman相关分析评价血清PDCD5水平与急性生理和慢性健康状况评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)积分、超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)、白细胞计数、中性粒细胞计数和年龄等因素的相关性。结果:在SIRS组、脓毒症组及重症脓毒症组中,血清PDCD5水平分别为(19.07±8.91)、(29.03±13.27)及(42.83±17.32)μg/L,显著高于健康对照组的(0.32±0.02)μg/L(P<0.05);血清PDCD5水平在SIRS组、脓毒症组及重症脓毒症组中呈现依次升高的变化规律,组间差异具有统计学意义(P<0.05)。此外,血清PDCD5水平与APACHEⅡ积分呈正相关(r=0.572,P<0.05)。结论:脓毒症患者血清PDCD5水平随着病情加重呈升高趋势,提示PDCD5表达上调在脓毒症发生、发展中可能起到一定作用。Objective： To investigate the changes of serum programmed cell death 5 （ PDCD5 ） levels in patients with critical illnesses including systemic inflammatory response syndrome （SIRS, except sep-sis）, sepsis （except severe sepsis） and severe sepsis. Methods： A total of 78 patients were enrolled in this study, of whom, 28 were with SIRS, 23 with sepsis and 27 with severe sepsis. Twenty-two healthy individuals were included as controls. The levels of serum PDCD5 were evaluated by enzyme-linked im-mune sorbent assay. And the correlation analyses were made in the levels of sreum PDCD5 and acute physiology and chronic health evaluation Ⅱ （ APACHE Ⅱ ） , high-sensitivity C-reactive protein （ hs- CRP）, white blood cell count, neutrophil count and age factors. Results： Serum PDCD5 levels in the SIRS, sepsis and severe sepsis groups were （19.07 ±8.91 ）, （29.03 ± 13.27） and （42.83 ± 17.32） μg/L respectively, which were significantly higher than that in the healthy control group （0.32 _± 0.02） μg/L. Serum PDCD5 levels in SIRS, sepsis and severe sepsis groups were gradually increased with significant difference at any in-between comparison （ P 〈 0.05 ）. Moreover, serum PDCD5 levels were positively correlated with the acute physiology and chronic health evaluation Ⅱ （APACHE Ⅱ ） score （ r = 0.572, P 〈 0.05 ）. Conclusion： The serum PDCD5 levels in the critically ill patients with sepsis were progressively increased with the worsened condition. The up-regulated expression of PDCD5 may play an important role in the development and progression of sepsis.

关 键 词：PDCD5蛋白 人 脓毒症 细胞凋亡 全身炎症反应综合征 

分 类 号：R631[医药卫生—外科学]