红树林植物木榄根际土壤真菌Penicillium sp. MA-37的化学成分研究  

Chemical constituents of Penicillium sp. MA-37,a fungus derived from rhizospheres of mangrove plant Bruguiera gymnorrhiza

作　　者：张一[1,2] 李晓明[1] 尚卓[1] 李春顺[1] 王斌贵[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《海洋科学》2013年第2期7-11,共5页Marine Sciences

基　　金：国家自然科学基金(30910103914)项目;科技部"973计划"项目(2010CB833802)

摘　　要：对采自海南文昌红树林根际土壤样品中分离到的真菌Penicillium sp.MA-37的次生代谢产物的化学成分进行了研究。利用硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、制备薄层层析以及重结晶等分离方法并通过现代波谱技术共分离、鉴定了8个天然产物,分别为:氮-(2-甲氧基-4-羟基苯)-丙酰胺酸(1),氮-(2,4-二甲氧基苯)-丙酰胺酸(2),氮-(2,4-二甲氧基苯)-丙酰胺酸甲酯(3),(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(4),(22E,24R)-麦角甾-4,6,8(14),22-四烯-3-酮(5),(22E,24R)-5α,8α-环二氧麦角甾-6,22-双烯-3β-醇(6),asperamide B(7),cis-4-hydroxy-6-deoxyscytalone(8);其中化合物1为新化合物,化合物2和3为新天然产物。From the fermentation culture of strain Penicillium sp.MA-37 derived from mangrove rhizosphere,eight compounds were isolated by separation techniques including column chromatography with silica gel and Sephadex LH-20,preparative TLC and re-crystallization.Structures of all purified compounds were elucidated by analysis of their spectroscopic data to be N-（2-methoxy-4-hydroxyphenyl）-malonamic acid（1）,N-（2,4-dimethoxyphenyl）malonamic acid（2）,N-（2,4-dimethoxyphenyl）-malonamic acid methyl ester（3）,（22E,24R）-ergosta-5,7,22-trien3β-ol（4）,（22E,24R）-Ergosta-4,6,8（14）,22-tetraen-3-one（5）,（22E,24R）-5α,8α-epidioxyergosta-6,22-dien-3β-ol（6）,asperamide B（7）,cis-4-hydroxy-6-deoxyscytalone（8）.Compound 1 was a new compound,while compounds 2 and 3 were reported as natural products for the first time.

关 键 词：红树林 根际土壤 新化合物 次生代谢产物 

分 类 号：Q58[生物学—生物化学]