两株硅藻筒柱藻和纤细角毛藻的油脂生产  

The Lipid accumulation of two oleaginous diatom species

作　　者：彭小伟[1] 张维[1] 刘天中[1] 

机构地区：[1]中国科学院生物燃料重点实验室,中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《海洋科学》2013年第2期43-47,共5页Marine Sciences

基　　金：国家科技支撑计划项目(2011BAD14B01);中国科学院重要方向性项目(KGCX-EW-309)

摘　　要：通过气泡柱光反应器评价了两株硅藻筒柱藻(Cylindrotheca fusiformis)和纤细角毛藻(Chaetocerosgracilis)的生物量、油脂及甘油三脂的积累。结果发现,其生物量与油脂产率相当,脂肪酸组成方面,也均以C16:0和C16:1为主,但是筒柱藻的油脂组成以甘油三酯为主,纤细角毛藻的油脂以单酰甘油酯、磷脂和糖脂为主。通过培养条件的优化,发现相比于降低初始氮元素与硅元素,降低培养液盐度,能够获得更高的生物量、油脂以及甘油三脂产率。通过筒柱藻批次培养最高获得了0.36 g/(L.d)的甘油三脂产率,且甘三酯占收获干物质的50%以上,具有相当好的应用潜力。The biomass,total lipids and Triacylglycerol contents of Cylindrotheca fusiformis and Chaetoceros gracilis were measured in batch,with bubble column photobioreactor.The results showed that the biomass and total lipid productivity were similar.The fatty acids composition of lipid from them were mainly C16：0 and C16：1,The composition of extracted lipid from C.fusiformis was mainly TAG,while in C.gracilis was mainly monoacylglycerol,phospholipids and glycolipids.Decreased the salinity of medium,the biomass,lipid prodcutivity,and TAG productivity were higher than which N or Si was limited.In these studies,TAG productivity can reach 0.36g/（L.d）,more than half of dry biomass.C.fusiformis has good potential capacity to be the microalgae species for microalgae biofuel production.

关 键 词：筒柱藻(Cylindrotheca fusiformis) 纤细角毛藻(Chaetoceros gracilis) 生物量 总脂含量 评价 

分 类 号：Q175[生物学—水生生物学]