盐度对拟穴青蟹幼蟹存活、生长和Na^+/K^+-ATP酶活性的影响  

Effect of salinity on the survival,growth and Na^+/K^+-ATPase activity of early juvenile mud crabs, Scylla paramamosain

作　　者：亓磊[1,2] 顾孝连[1] 蒋科技[1] 乔振国[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]长江上游特有鱼类国家级自然保护区赤水增殖放流站,贵州遵义563000

出　　处：《海洋科学》2013年第2期56-60,共5页Marine Sciences

基　　金：上海市科委项目(10JC1418600;12DZ1909303);中央级公益性科研院所基本科研业务费专项资金资助项目(2011M10)

摘　　要：研究了盐度(6、13、20、27和33)对拟穴青蟹(Scylla paramamosain)幼蟹生长发育和Na+/K+-ATP酶活性的影响。结果表明,盐度对拟穴青蟹幼蟹甲宽和体质量特定生长率、C3蜕壳间期、甲宽增量(C1至C2和C2至C3)及Na+/K+-ATP酶活性有显著影响(P<0.05),但对幼蟹存活率、C2蜕壳间期及C3至C4甲宽增量无显著影响(P>0.05)。盐度13处理组特定生长率最高、蜕壳间期最短,盐度6处理组特定生长率最低,但与盐度20、27和33处理组无显著差异(P>0.05),而盐度33处理组蜕壳间期最长;第一次蜕壳后盐度20处理组甲宽增量最大,后两次蜕壳盐度13处理组均是最大;盐度处理0.5、4、7和11 d时,Na+/K+-ATP酶活性分别表现为盐度6>13>20>27>33、6>13>27>20>33、6>13>20>27>33和6>13>33>20>27的趋势,低盐环境下Na+/K+-ATP酶活性的升高有助于拟穴青蟹幼蟹对低渗环境的适应。The effects of low salinities on development and Na＋/K＋-ATPase activity of early juvenile mud crabs（Scylla paramamosain） were studied.The salinities tested for the experiment were 6,13,20,27 and 33.The results showed that salinity significantly affected the specific growth rates（SGR） for carapace length or weight,C3 intermolt duration,carapace width increase at C1 to C2 or C2 to C3 stages and Na＋/K＋-ATPase activity of early juvenile mud crabs（P0.05）.There was no significant difference in survival,C2 intermolt duration and carapace width increase at C3 to C4 stages among salinities tested（P0.05）.The molth were delayed and intermolt durations were extended at 33,and then the growth was inhibited.At 13,maximum SGR for carapace length or weight was achieved,and at the same time,intermolt duration was relatively shortest for all salinities tested at C2 and C3 stages.At 6,the carapace width increased at the first molt and C2 to C3stages was the least among salinities tested.In each time point,the enzyme activity became elevated in order to accommodate to low salinities.Base on the above,it is suggested that early juvenile S.paramamosain tolerate a broad range of salinity,and the low salinity（13） can result in the best growth and shortest intermolt duration.However,from an aquaculture point of view,a salinity range of 13 - 27 was recommended at C1to C3stages for early juvenile S.paramamosain,which widened（6 - 27） in the later stages for the culture.

关 键 词：拟穴青蟹(Scylla paramamosain) 幼蟹 盐度 存活 生长 Na+ K+-ATP酶活性 

分 类 号：S968.251[农业科学—水产养殖] Q175[农业科学—水产科学]