耐辐射奇球菌未知功能基因DR_2566的克隆与生物信息学分析  

Cloning and bioinformatics analysis of an unknown-function gene of DR_2566 in Deinococcus radiodurans

作　　者：何彬彬[1] 李斌元[2] 马云[2] 杨杰[1] 王三虎[2] 苏泽红[2] 唐艳[1] 何淑雅[1,2] 

机构地区：[1]南华大学公共卫生学院,衡阳421001 [2]南华大学生物化学与分子生物学教研室,衡阳421001

出　　处：《辐射研究与辐射工艺学报》2013年第2期1-6,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(81272993);湖南省研究生科研创新项目(CX2012B386)资助

摘　　要：克隆与辐射抗性相关但功能未知的新基因DR_2566,并应用生物信息学初步探讨其功能。应用PCR技术从耐辐射奇球菌基因组中扩增DR_2566基因全长ORF,选用pGEM-T载体进行TA克隆,通过限制性酶切及测序进行鉴定。应用生物信息学初步分析其染色体定位、蛋白序列、结构域及功能。成功克隆了耐辐射球菌未知功能基因DR_2566,生物信息学分析显示此基因全长为507 bp,编码168个氨基酸,其编码蛋白的理论分子量为19.136KDa,三级结构预测显示DR_2566蛋白质具有短修复内切酶性质。成功克隆了耐辐射奇球菌基因DR_2566全长ORF,生物信息学预测DR_2566蛋白可能具有错配修复作用。The objective of this study was to clone DR_2566 and to investigate its function preliminary using bio-informatics. Full-length open reading frame （ORF） of DR_2566 gene was amplified by PCR, and pGEM-T was chosen as a vector for gene TA cloning. After ligation, restriction endonuclease analysis and sequencing were carried out for identification. On the other hand, bio-informatics was used to analyze the localization of the target gene on the chromosome, speculate protein sequence, domain and function. DR_2566 gene was cloned successfully. Bio-infor- matic analysis results show that the target gene, with 507 bp full-length, encodes a protein with 168 amino acids and the theoretical molecular mass of this protein is about 19.136 KDa. Tertiary structure predicting indicates DR_2566 protein is a kind of endonuclease that can repair short DNA damage fragment. Full-length ORF of DR_2566 in D. Radiodurans was cloned successfully. Bio-informatic analysis indicated the DR_2566 protein might be involved in mismatch repairing function in D. Radiodurans.

关 键 词：耐辐射球菌 DR_2566基因 基因克隆 生物信息学 

分 类 号：Q527[生物学—生物化学] Q78