多房棘球蚴不同方法接种灰仓鼠感染效果观察  

Observation on Infectiousness of Echinococcus multilocularis by Different Inoculation Methods in Cricetulus migratorius

作　　者：廖力夫 刘斌 徐艺玫 燕顺生 史深 

机构地区：[1]新疆实验动物研究中心,乌鲁木齐830002

出　　处：《实验动物科学》2013年第1期22-25,共4页Laboratory Animal Science

基　　金：国家"十一五"科技支撑计划重点项目(No.2009BA183B02)

摘　　要：目的观察将多房棘球蚴(Echinococcus muhilocularis简称Em)接种灰仓鼠腹腔、前肢腋下、心脏和肝脏组织的感染效果,为探索建立多组织或器官包虫病动物模型提供依据。方法按1000个头节/只剂量经腹腔和前肢腋下接种灰仓鼠,经腹膜穿刺肝脏和经胸腔穿刺心脏接种灰仓鼠,按计划解剖检查包囊发育状况和包囊寄生部位,计算包囊系数(包囊质量/体质量×100%)和感染率。结果 100 d处理腹腔接种29只,腹腔100%感染,包囊系数7.1%,300 d处理7只,除腹腔都有包囊寄生外,包囊浸润肝脏3只,浸润脾脏和肾脏各2只,总包囊系数51.7%。前肢腋下接种的分别于116 d和290 d处理19只和5只,腋下均有包囊寄生,包囊系数分别为3.27%和22.95%。100d处理肝脏接种的17只,共10只有包囊,包囊系数7.1%,包囊寄生部位为腹腔8只,肝脏5只,心脏1只,肺部3只。115 d处理心脏接种26只,包囊寄生部位为心脏6只,肺部9只,胸腔1只,其中有两只同时心脏和肺脏寄生包囊,共13只寄生包囊,包囊系数2.91%。结论腹腔环境适宜Em生长发育,适合做周期短的动物模型,接种300 d包囊有向实体器官浸润的现象。Em在前肢腋下发育缓慢,适合做Em保种。由于动物模型中的心脏和肝脏接种感染率低,接种技术和方法需改进,以便提高感染率。Objective To observe infectiousness of Echinococcus multilocularis by different inoculation methods in Cricetulus migratorius so as to provide the foundation for developing hydatid disease animal model in variety of tissues and organs. Method 1 000 scoleces per Cricetulus migratorius were inoculated into armpit, abdominal cavity, heart or liver. The development status and parasitic parts of the cysts were surveyed by dissection, and cysts coefficient （cyst weight / body weight × 100% ） and infection rates were computed. Result The infection rate in 29 animals was 100% with a cyst coefficient of 7.1% at 100 days after intraperitoneal inoculation. The cyst parasitism in the abdominal cavity was found in all 7 sacrificed animals, and liver cyst infiltration was found in 3 animals, spleen cyst infiltration in 2 animals and kidney cyst infiltration in 2 animals with total cyst coefficient of 51.7% at 300 days after intraperitoneal inoculation. At 116 days after armpit inoculation, the cyst parasitism in armpit was revealed in all 19 sacrificed animals with a cyst coefficient of 3.27% , and at 290 days after armpit inoculation the cyst parasitism in armpit was revealed in all 5 sacrificed animals with a cyst coefficient of 22.95%. At 100 days after liver inoculation in 17 animals, the cyst parasitism in the abdominal cavity revealed in 8 animals, the cyst parasitism in liver in 5 animals, the cyst parasitism in heart in 1 animal, and the cyst parasitism in lung in 3 animals with a cyst coefficient of 7. 1%. At 115 days after heart inoculation in 26 animals, the cyst parasitism in heart revealed in 6 animals, the cyst parasitism in lung in 9 animals, and the cyst parasitism in thorax in 1 animal with a cyst coefficient of 2.91%. Conclusion The celiac environment of Cricetulus migratorius may be hospitable for the growth and development of Em, which is appropriate for developing of the short-period animal model of hydatid disease. Em grows and develops slowly in the forelimb armpit of Cricetulus migratorius, so that

关 键 词：多房棘球蚴(Em) 灰仓鼠 动物模型 包囊系数 器官 

分 类 号：S858.31[农业科学—临床兽医学]