棉花植物同源结构域(PHD)转录因子在非生物胁迫中的表达分析  

Expression of Cotton(Gossypium hirsutum L.) Plant Homeodomain Transcription Factors(PHD) under Abiotic Stress

作　　者：杨雷[1,2] 谢宗铭[2] 司爱君[2] 田琴[2] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832000 [2]新疆农垦科学院农业分子技术育种中心/作物种质创新与基因资源利用兵团重点实验室.新疆石河子832000

出　　处：《新疆农业科学》2013年第3期409-416,共8页Xinjiang Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2009ZX08009-090B);兵团博士资金项目(2008JC03)

摘　　要：【目的】通过对陆地棉(Gossypium hirsutum L.)PHD(Plant homeodomain)转录因子在棉花不同组织和非生物胁迫条件下的表达分析,筛选棉花逆境胁迫应答基因。【方法】根据已报道的28个棉花GhPHD基因的EST序列合成了特异引物,使用半定量RT-PCR方法检测GhPHD在棉花种子、根、茎、叶、纤维(15DPA)中的表达以及在干旱、高盐(150 mmol/L NaCl)、低温(4℃)非生物胁迫下的表达。【结果】棉花PHD转录因子在各组织中的表达存在差异,85.7%的基因在种子中表达,在纤维中表达的占35.7%。在干旱胁迫条件下GhPHD1、GhPHD6和GhPHD22呈上调表达,在高盐胁迫条件下GhPHD1、GhPHD16呈上调表达,低温胁迫激活GhPHD9表达,其它基因对三种非生物胁迫无明显应答。【结论】部分GhPHDs在陆地棉应答和适应非生物胁迫过程中可能发挥重要的作用。[ Objective] In order to identify novel genes involved in upland cotton （Gossypium hirsutum L. ）, plant stress adaptation, expression of GhPHDs under different kinds of abiotic stresses, such as drought, high salt and low temperature were analyzed. [ Method ] According to the reported 28 GhPHDs, specific primers were synthesized. Semi - quantitative RT - PCR method was used to analyze expression patterns of GhPHDs in cotton seed, root, stem, leaf and fiber（15DPA） and under abiotic stresses such as drought, high salt （150 mmol/L NaCI） and low temperature （4~C）. [ Result] The 28 GhPHDs showed different tissue - specific expression patterns in seed, root, stem, leaf and fiber（15DPA） of cotton. The proportion of genes expressed in seed was 85.7% and 35.7% in fiber. These genes were differentially regulated by various abiotic stresses. The expressions of GhPHD1, GhPHD6 and GhPHD22 were activated by drought stress, and GhPHD1, GhPHD16 were up - regulated by high salt stress. GhPHD9 was up - regulated by low temperature. Other genes didnt show obvious response to these three abiotic stresses. [ Conclusion] It implies that GhPHDs may play important roles during upland cotton plant adapted to abiotic stresses.

关 键 词：GhPHDs 非生物胁迫 组织差异表达 半定量RT-PCR 

分 类 号：S562[农业科学—作物学] S188