高效液相色谱法同时测定蒙药齿缘草中槲皮素和山奈素的含量  

SIMULTANEOUS DETERMINATION OF QUERCETIN AND KAEMPFEROL IN ERITRICHII HERBA BY HPLC

作　　者：巴俊杰[1] 布仁[1] 马丽娟[1] 苗娜[1] 贾心语 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010110

出　　处：《内蒙古医科大学学报》2013年第2期132-135,共4页Journal of Inner Mongolia Medical University

基　　金：国家药典委员会2010年度药品标准提高项目(453)

摘　　要：目的:建立蒙药齿缘草中槲皮素和山奈素含量的测定方法。方法:采用高效液相色谱法。色谱柱以十八烷基硅烷键合硅胶为填充剂;流动相为甲醇～0.4%磷酸溶液(45∶55);检测波长为368 nm;流速为1.0mL/min.结果:槲皮素在31.4～314 ng范围内有良好的线性关系(r=0.9997),平均回收率为96.96%,(RSD=1.54%);山奈素在30～300 ng范围内有良好的线性关系(r=0.9999),平均回收率为97.92%(RSD=1.91%)。结论:该方法简便,准确,重复性好,可作为齿缘草中槲皮素和山奈素含量的测定方法。Objective： To establish a determination method of quercetin and kaempferol in Eritrichii Herba. Metbods：HPLC was applied. The column was Eighteen alkyl silane bonded silica gel, the mobile phase was methanol -0.4% phosphoric acid（45 ： 50） ,the flow rate was 1.0 mL/min and the detection wavelength was 368nm. Result： The linear range of Quercetin was 31.4 - 314 ng （ r = 0.9997 ） ,the average recovery was 96.96% （ RSD = 1.54% ）. The linear range of kaempferol was 30 - 300 ng（ r = 0.9999 ）, the average recovery was 97.92% （ RSD = 1.91% ）. Conclusion：The method is simple, accurate, reproducible and can be used for the determination of quercetin and kaempferol in Eritrichii Herba.

关 键 词：齿缘草 槲皮素 山奈素 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]