胰岛素对糖尿病大鼠肺组织基质金属蛋白酶2表达的影响  

Effect of insulin on the expression of matrix metalloproteinase-2 in lung tissue of diabetic rat

作　　者：周训美[1] 赵倩[1] 李建[1] 陈袁[1] 李显文[1] 

机构地区：[1]遵义医学院附属医院内分泌科,贵州遵义563099

出　　处：《遵义医学院学报》2013年第1期25-28,共4页Journal of Zunyi Medical University

摘　　要：目的观察胰岛素对不同糖尿病病程大鼠肺组织内基质金属蛋白酶2(MMP-2)表达的影响,探讨胰岛素对糖尿病大鼠肺组织的保护作用。方法 60只SD大鼠随机分为对照组、糖尿病组、胰岛素组;用持续高糖、高脂饲料喂养加腹腔注射链脲佐菌素(STZ 40mg/kg,ip)的方法建立糖尿病大鼠模型。成模后胰岛素组每日皮下注射精蛋白锌胰岛素控制血糖。观察成模后第4周、8周各组大鼠肺组织形态学及MMP-2的表达。结果糖尿病大鼠肺组织出现大量炎症反应、肺间质增生。第8周糖尿病大鼠肺组织内MMP-2(30.5±1.4)表达较同期对照组(4.6±0.8)升高(P<0.05)。第8周胰岛素组大鼠肺组织内MMP-2(5.6±1.6)表达较同期糖尿病组降低(P<0.05)。结论①糖尿病可引起大鼠肺组织损伤;②糖尿病大鼠肺组织内MMP-2表达增高;③精蛋白锌胰岛素可减少糖尿病大鼠肺组织内MMP-2的表达,减轻糖尿病所致肺组织损伤。Objective To observed the effect of insulin on the expression of matrix metalloproteinase - 2 （ MMP - 2） in lung tissue of diabetic rats with different course of DM, and explore the protective effect of insulin on the lungs in diabetic rat. Methods 60 sprague dawley male rats were randomly divided into control group, diabetic group and insulin group. The diabetic rats was established by feeding food with high fat and sugar, and then administrated with streptozotocin （40 mg/kg, ip）. The rats in insulin group were daily injected protamine zinc insulin subcutaneously. The changes on morphology and MMP -2 ex- pression of lung tissue in each group were analyzed at four weeks and eight weeks. Results There were obvious inflammatory reaction and pulmonary stromal hyperplasia in lung tissue of diabetic rats. At eight weeks,the expression of MMP -2 in lung tissue of diabetic rats were higher than that in control group （ P 〈 0. 05 ）, However, insulin treatment could significantly reduce the inflammatory reaction and pulmo- nary stromal hyperplasia, as well as the expression of MMP - 2 in lung tissue （ P 〈 0. 05 ）. Conclusion ① Diabetes can cause pathological injury of rat lung. ②The expression of MMP - 2 was increased in lung tissue of diabetic rats. ③Protamine zinc insulin can reduces the expression of MMP - 2 in the lung tissue of diabetic rats and alleviate diabetes - induced lung pathological damage.

关 键 词：基质金属蛋白酶2 糖尿病 胰岛素 肺 

分 类 号：R473.5[医药卫生—护理学]