人外周血清血浆对小鼠红系造血祖细胞集落形成的影响  

Effect of Human Peripheral Blood Serum and Plasma on CFU Yields of Hematopoietic Progenitor Cells of Mouse Bone Morrow

作　　者：刘艳霞[1] 沙珍[2] 郑俊[2] 次仁布藏 张世馥[3] 刘舒[1] 裴澍萱[2] 

机构地区：[1]北京市心肺血管疾病研究所 [2]西藏自治区第二人民医院 [3]中国医学科学院基础医学研究所

出　　处：《西藏医药》2000年第4期12-13,共2页Tibetan Medicine

基　　金：国家自然科学基金!(NO 396 6 0 0 2 8);卫生部联合支持项目

摘　　要：目的　通过研究人外周血清血浆对小鼠红系造血祖细胞集落形成的影响 ,补充完善小鼠红系造血祖细胞体外培养的方法研究。方法 :采用细胞培养的方法以红系集落形成单位 (CFU—E) ,暴裂样集落形成单位 (BFU—E)为指标 ,探讨红系造血祖细胞体外培养的影响因素。结果和结论 :( 1 )正常生理浓度的ED TA、Ca、血清对红系造血祖细胞集落形成没有明显作用。 ( 2 )外周血血浆及纤维蛋白原 (Fibrinogen ,Fib)能降低红系造血祖细胞集落形成。Objective:To explore the effect of human peripheral blood serum and plasma on CFU yields of hematopoietic progenitor cells of mouse bone morrow,complement and perfect the methodology of hematopoietic progenitor cells of mouse bone morrow in vitro culture.Methods:The effects of human peripheral blood serum and plasma on CFU-E and BFU-E yields of hematopoietic progenitor cells of mouse bone morrow was studied.Results and Conclusions:(1)EDTA.Ca of normal physiological concentration and serum showed no effect on CFU-E of BFU-E yields of hematopoietic progenitor cell of mouse bone morrow.(2)Fibrinogen and plasma of peripheral blood significantly inhibited CFU-E yields of hematopoietic progenitor cell of mouse bone morrow.

关 键 词：体外培养 CFU-EBFU-E 红系造血祖细胞 

分 类 号：R446.1[医药卫生—诊断学]