褪黑素减轻丙烯酰胺对大鼠小脑的氧化损伤作用  

Alleviation of acrylamide-induced oxidative damage by melatonin in rat's cerebellum

作　　者：熊飞[1] 朱兰兰[1] 严汉英 郭雄雄[1] 严红[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030 [2]武钢安全环保部安全环保研究所

出　　处：《毒理学杂志》2013年第1期23-26,共4页Journal of Toxicology

基　　金：国家自然科学基金项目(30771822)

摘　　要：目的探讨褪黑素(Melatonin,MT)是否能减轻丙烯酰胺(ACR)导致的大鼠小脑的氧化损伤。方法 52只SD雄性大鼠按体重随机分为4个组,每组13只,分别为对照组、ACR组、MT组、MT+ACR联合组,均单笼饲养。对照组灌胃溶剂生理盐水,ACR组灌胃40 mg/kg ACR溶液,MT组腹腔注射5 mg/kg MT,MT+ACR联合组灌胃40 mg/kgACR溶液同时腹腔注射5 mg/kg MT,连续12 d。染毒结束后,断头处死大鼠,在冰盘上分离小脑,对小脑进行苏木素-伊红(HE)染色、氧化损伤检测和DNA损伤检测。结果 ACR组出现浦肯野细胞核固缩等异常改变,丙二醛(MDA)含量明显升高(P<0.05),T-SOD活性和GSH含量明显下降(P<0.05,P<0.01),彗星试验中尾长,尾部DNA百分含量及Olive尾距均显著增加,差异均有统计学意义(P<0.05或P<0.01)。MT+ACR组小脑结构正常,与ACR组相比,MT+ACR组MDA含量、彗星试验尾长、尾部DNA百分含量、Olive尾距均明显下降,T-SOD活性和GSH含量明显升高,差异均有统计学意义(P<0.05或P<0.01)。结论 ACR可以引起大鼠小脑结构改变、氧化损伤和DNA损伤,MT可以减轻ACR对小脑的结构损伤和氧化损伤。Objective To investigate whether melatonin（MT） could alleviate the oxidative damage of acrylamide（ACR） in rat cerebellum.Methods Fifty two SD male rats were randomly divided into four groups which are Control group,ACR group,MT group,MT＋ACR joint group,all single-cage rearing,each group with thirteen animals in each group.The control group was given normal saline,ACR group was given 40 mg/kg ACR solution by oral gavage,MT group was intraperitoneal injected with 5 mg/kg MT,MT＋ACR joint group was given 40 mg/kg ACR by oral gavage and 5 mg/kg MT by intraperitoneal injection.After 12 days exposure,the rats were decapitated,the cerebellum were separated on the ice tray,and used for HE staining,detection of oxidative damage and DNA damage.Results ACR group showed Purkinje cell shrinkage and other abnormal changes.MDA content of ACR group was significantly higher（P＆lt;0.05）,T-SOD activity and GSH levels decreased significantly（P＆lt;0.05,P＆lt;0.01）,Comet assay was expressed as tail length,tail DNA and Olive tail moment percentage content which increased significantly（P＆lt;0.05 or P＆lt;0.01）.The cerebellum structure of MT＋ACR joint group was normal.Compared with the ACR group,MDA content of the MT＋ACR Group was significantly decreased,T-SOD activity and GSH levels increased significantly,comet assay showed tail length,the tail DNA percentage and Olive tail moment significantly decrease,and there were significant differences between then（P＆lt;0.05 or P＆lt;0.01）.Conclusion ACR could cause structural changes,oxidative stress and DNA damage.MT could alleviate those cerebellum damage induced by ACR in rats.

关 键 词：褪黑素 丙烯酰胺 小脑 氧化损伤 

分 类 号：R994.6[医药卫生—毒理学]