骨髓基质干细胞片层复合聚乳酸羟基乙酸支架构建组织工程骨  

Constructive tissue engineering bone with BMSC sheets and PLGA scaffold

作　　者：姚超[1] 李宁毅[1] 刘风芝[1] 王玲玲[1] 于跃远[1] 隋爱华[1] 

机构地区：[1]青岛大学医学院附属医院口腔科,266003

出　　处：《中华医学美学美容杂志》2013年第2期118-121,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：基金项目：国家自然科学基金资助项目（编号：30872896）;山东省自然科学基金资助项目（编号：Y2008C77）

摘　　要：目的制备骨髓基质干细胞片层，探讨其对组织工程骨成骨的作用。方法培养骨髓基质干细胞（BMSCs）并向成骨方向诱导，利用温度反应性培养皿制备骨髓基质干细胞片层，光学及电子显微镜下观察其形态。将经过预处理的三维多孔聚乳酸羟基乙酸（PLGA）支架注射BMSCs悬液并复合干细胞片层后植入犬左侧下颌骨缺损，为实验侧；将未复合细胞片层的支架植入右侧．为对照侧。16周后，对新生骨行影像学及组织学观察分析。结果片层中细胞排列紧密，生长活跃。实验侧吸光度值高于对照侧（P〈0．05），并可见较多哈弗系统及板层骨样结构。结论经温度反应性培养皿制备的干细胞片层结合PLGA支架可形成具有板层状结构的组织工程骨。Objective To construct bone marrow stem cell sheets and to investigate its effects in the process of osteogenesis. Methods BMSCs were differentiated into osteoblasts and then seeded into a temperature responsive culture dish to construct BMSC sheets. PI.GA scaffolds in which both BMSC suspension and BMSC sheets were added, were implanted into the left side of the dogs＇ mandible. In the other side, PLGA scaffolds that were not wapped with BMSC sheets were implanted as control. At 16 weeks, the samples were processed for radiological analysis and histological examination. Results Cells in the BMSC sheets grew well. In the experimental side, the optical density of the samples was higher than that of the control side （P〈0.05） and plenty of lamellar bones and Haversian system were observed. Conclusions The formation of lamellar bones can be promoted by PLGA scaffolds and BMSC sheets in the process of tissue engineering bone reconstrution.

关 键 词：骨髓基质干细胞 细胞片层 温度反应性培养皿 聚乳酸羟基乙酸 组织工程骨 

分 类 号：R318.08[医药卫生—生物医学工程]