结缔组织生长因子基因沉默对人体瘢痕疙瘩移植动物模型中Ⅰ型胶原表达的影响  

Effects of CTGF gene silencing on expression of type Ⅰ collagen in a nude mouse model

作　　者：杜勇[1] 金培生[1] 张爱君[1] 陶常波[1] 李雪阳[1] 李强[1] 沈才齐[1] 

机构地区：[1]徐州医学院附属医院整形外科,221002

出　　处：《中华医学美学美容杂志》2013年第2期126-129,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：基金项目：江苏省卫生厅资助项目（编号：z200705）

摘　　要：目的探讨利用RNA干扰（RNA interference，RNAi）技术特异沉默裸鼠人体瘢痕疙瘩（KD）移植模型中的结缔组织生长因子（connective tissue growth factor，CTGF），为KD的基因治疗提供依据。方法构建裸鼠人体KD移植动物模型，利用扩增好的质粒对动物模型进行体内转染，逆转录聚合酶链反应（RT-PCR）分析实验组与对照组CTGF硬Ⅰ型胶原mRNA表达差异，免疫组织化学染色分析CTGF及Ⅰ型胶原蛋白表达差异。结果实验组CTGF mRNA及蛋白表达量均显著降低（P〈0．05），体内转染可显著抑制CTGF表达；实验组Ⅰ型胶原mRNA及蛋白表达量显著降低（P〈0．05）。且Ⅰ型胶原与CTGF的表达量呈正相关（r=0．979）。结论体内环境下抑制CTGF的表达可降低KD中Ⅰ型胶原的水平，CTGF体内转染可望影响Ⅰ型胶原的生成，从而抑制KD的生长。Objective To investigate the specific silencing of connective tissue growth factor （CTGF） in a nude mouse keloid model, using RNA interference （RNAi） technique, and to provide the basis for gene therapy of keloid. Methods The nude mouse keloid model was established, and then transfected in vivo with well amplifiating plasmid. The mRNA expression levels of CTGF mRNA and type Ⅰ collagen mRNA were detected with reverse transcription polymerase chain reaction （RT-PCR）. The distribution and protein expression levels of CTGF and type Ⅰ collagen were determined quantita tively using immunohistochemistry. Results The expression of CTGF at mRNA and protein levels was decreased in the experiment group, and the expression of type I collagen at rnRNA and protein levels was also decreased after transfection, as compared with negative control group and blank group, with significant difference between groups （P〈0. 05）. Moreover, the expression of type Ⅰ collagen and CTGF was positively correlated （r=0. 979）. Conclusions Keloid type Ⅰ collagen can he decreased through in vivo inhibiting CTGF expression. The transfection of CTGF gene in vivo may have effects on type Ⅰ collagen generation, and thus inhibit the keloid growth.

关 键 词：瘢痕疙瘩 RNA干扰 结缔组织生长因子 体内干预 动物模型 

分 类 号：R6[医药卫生—外科学] R-332[医药卫生—临床医学]