重组人生长激素对THP-1单核细胞TNF-α和IL-6分泌的影响与NF-κB活性的相关性研究  

Effect of recombinant human growth hormone on the secretion of TNF-α and IL-6 by THP-1 cells and its correlation with the NF-κB pathway

作　　者：张瑞芹[1] 谭静[2] 张国俊[3] 石如玲[3] 宋向凤[3] 毛兰芝[3] 

机构地区：[1]新乡医学院护理学院,河南新乡453003 [2]新乡医学院第三附属医院,河南新乡453003 [3]新乡医学院基础医学院,河南新乡453003

出　　处：《细胞与分子免疫学杂志》2013年第4期368-371,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河南省高校骨干教师资助(2009GGJS-086)

摘　　要：目的探讨重组人生长激素(rhGH)对THP-1单核细胞TNF-α和IL-6分泌的影响,并研究其与NF-κB活性的相关性。方法应用ELISA检测rhGH诱导THP-1细胞培养上清中TNF-α和IL-6的分泌情况,并观察LPS和NF-κB特异性抑制剂BAY11-7082对其分泌的影响;应用荧光素酶报告基因和电泳迁移率实验(EMSA)检测NF-κB在rhGH诱导的单核细胞中的活化程度。结果 rhGH单独可以促进THP-1细胞分泌TNF-α和IL-6,抑制LPS诱导的细胞因子的分泌;BAY11-7082能显著抑制rhGH和LPS诱导的TNF-α和IL-6的分泌,NF-κB活性与TNF-α和IL-6的分泌呈现明显的相关性。结论 rhGH可通过NF-κB信号通路双向调节THP-1单核细胞TNF-α和IL-6的分泌。Objective To investigate the effect of recombinant human growth hormone （rhGH） on the secretion of TNF- α and IL-6 in THP-1 ceils and analyze the correlation between the secretion and NF-κB signaling pathway. Methods The concentrations of TNF-α and IL-6 in the supernatants of THP-1 induced by rhGH were measured with ELISA. The effects of LPS and an NF-KB inhibitor, BAYll-7082 on the secretion of TNF-α and IL-6 were also observed. The activity of NF-KB was detected by luciferase reporter and electrophoretic mobility shift assay （EMSA） in THP-1 cells induced by rhGH. Results rhGH alone promoted the secretion of TNF-α and IL-6 in THP-1 cells while inhibited the secretion of TNF-α and IL-6 in THP- 1 stimulated by LPS. BAYlI-7082 inhibited the secretion of TNF-α and IL-6 in THP-1 stimulated by LPS and rhGH. The activity of NF-κB was significantly correlated with the secretion of TNF-α and IL-6. Conclusion rhGH played a bidirectional role in the regulation on the secretion of TNF-α and IL-6 via NF-κB signaling pathway in THP-I cells.

关 键 词：重组人生长激素 核因子KB 肿瘤坏死因子Or 白细胞介素6 

分 类 号：R392.11[医药卫生—免疫学] Q256[医药卫生—基础医学]