肝原位移植瘤裸鼠模型的建立及活体荧光成像检测  

Establishment of an intrahepatic xenograft tumor model in nude mice and its detection by in vivo fluorescence imaging system

作　　者：贾小飞[1,2] 闫博[1,2] 张熠杰[3] 蒋帅[4] 赵晶[1,2] 杨安钢[1,5] 

机构地区：[1]第四军医大学国家肿瘤生物学重点实验室,陕西西安710032 [2]第四军医大学生物化学与分子生物学教研室,陕西西安710032 [3]第四军医大学口腔医学系,陕西西安710032 [4]第四军医大学航空与航天医学系,陕西西安710032 [5]第四军医大学免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2013年第4期426-429,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家科技重大专项(2008ZX10002-023);国家自然科学基金(30873006;81172149;81201777)

摘　　要：目的建立能够通过活体荧光成像系统实时监测肿瘤进展的肝原位移植瘤裸鼠模型。方法重组质粒pcDNA3.1-Luc转染细胞构建稳定表达荧光素酶的PLC/PRF/5肝癌细胞株,将该细胞注入裸鼠肝脏实质内,应用活体成像技术动态监测肿瘤进展情况,解剖荧光信号阳性裸鼠观察肿瘤组织生长情况。免疫荧光检测肿瘤组织中HBsAg表达。结果对裸鼠肝原位移植瘤应用活体荧光系统成像,在肿瘤细胞注射部位检测到荧光信号,解剖动物后可在肝脏组织中观察到异质细胞团块。免疫荧光证实移植瘤中有HBsAg表达。结论肝脏原位移植瘤裸鼠模型建立成功,为抗肝癌药物的研发提供了工具。Objective To establish an intrahepatic xenograft tumor model in nude mice and dynamically monitor the tumor growth by in vivo fluorescence imaging system. Methods We first transfected the recombinant plasmid pcDNA3, l-Luc to constructe the PLC/PRF/5 cell line which stably expressed luciferase, and then injected the cell line into the liver of nude mice. Tumor growth was monitored dynamically using in vivo fluorescence imaging system. The fluorescence-positive mouse was dissected to observe the tumor. Immunofluoresence histochemistry was used to detect the expression of HBsAg in Xenografe. Results In the nude mouse model bearing intrahepatic xenograft tumor, robust fluorescence was detected where the PLC/PRF/5 cells were injected. Xenograft was observed in the liver by dissecting the fluorescence-positive mouse. The expression of HBsAg in xenograft tissues was confirmed. Conclusion Intrahepatic xenograft tumor model in nude mice has been established successfully, which offers a tool for therapeutic development and evaluation of anti-hepatocelluar carcinoma drugs.

关 键 词：肝原位移植瘤裸鼠模型 活体荧光成像 荧光素酶 PLC PRF 5细胞株 

分 类 号：R392.33[医药卫生—免疫学] R735.7[医药卫生—基础医学]