发酵、梨支原体感染小鼠小肠黏膜和胰腺组织细胞电镜观察  

Electron microscope observation on mouse intestinal mucosa and pancreatic tissue cells induced by Mycoplasma fermentans and Mycoplasma pirum

作　　者：管瑜[1] 沈丹丹[1] 周丽萍[1] 

机构地区：[1]温州医学院检验医学院和生命科学学院,温州325035

出　　处：《中国人兽共患病学报》2013年第4期361-365,共5页Chinese Journal of Zoonoses

基　　金：温州市科技计划项目(Y20110107)~~

摘　　要：目的探讨发酵支原体(Mycoplasma fermentans,Mf)和梨支原体(Mycoplasma pirum,Mpi)感染致死小鼠小肠黏膜和胰腺组织细胞超微结构病理改变。方法 35只清洁级ICR小鼠,隔日腹腔注射环磷酰胺(50mg/kg.d)5次制成免疫抑制模型。分为Mf、Mpi感染组(n=12)和生理盐水(NS)对照组(n=11),分别腹腔注射0.3mL标准菌株Mf、Mpi(6×108-9/mL)和无菌生理盐水,计算各组死亡率,取小鼠血液进行支原体再培养以及感染12h、6d后取小肠黏膜和胰腺组织超微结构观察。结果免疫抑制小鼠Mf或Mpi感染6d后,全部死亡,支原体再培养阳性。电镜观察显示:Mf或Mpi感染12h后,小肠黏膜和胰腺组织细胞即表现超微结构病理改变,感染6d后,小肠黏膜组织细胞显著水肿,肿胀,线粒体空泡,胰腺细胞线粒体空泡,酶原颗粒变小,细胞变性、坏死,与NS组比较差异显著,且胰腺组织细胞病理改变比小肠黏膜明显。结论发酵支原体或梨支原体感染小鼠12h后,小肠黏膜和胰腺组织细胞即表现超微结构病理改变,6d后出现细胞水肿、变性及坏死。且胰腺组织细胞病理改变比小肠黏膜组织细胞更明显。To investigate the Mycoplasma fermentans （Mf） and Mycoplasma pirum （Mpi） infection and the ultra- structure pathology change of pancreas tissue in mouse intestine,35 ICR mice were made for immunosuppression models by in- traperitoneal injection of cyclophosphamide for five times every other day and mice were divided into Mf infection group （n=12）, Mpi infection group （n= 12）, and the NS control group （n= 11）. They were respectively received 0. 3 mL （6 ×108-9/mL） standard strains of Mf and Mpi and the sterile saline by intraperitoneal injection to evaluate the mortality rates in each group. The serum samples from each group of mice were taken for re-cultivation of the Mycoplasma. And the intestinal mueosa and pancreatic tissue of the mice were subjected to ultrastructural observation by electron microscope after 12 hours and 6 days infection, respectively. All of the mice in Mf and Mpi infection groups were dead after infection for 6 days, and the Mycoplasma re-cultivation was positive. Ultrastructural observations indicate that after 12 hours of Mf or Mpi infection, in- testinal mucosa and pancreatic tissue cell had ultrastructurally pathological changes. In the 6th day after infection, significantly edema, swelling of mitochondria, vacuoles, mitochondria vacuoles of pancreatic cells, zymogen granules degeneration, and nec- rosis could be observed in the small intestinal mucosa cells, which showed significant difference compared with those in the NS group. And the pancreas tissue pathological change is more serious. The experiment results indicate that intestinal mucosa and pancreatic tissue cell change the ultrastructural pathology afte 12 hours of Mf or Mpi infection. After 6 days, edema, denatur- ation and necrosis could be observed in the cells, and the pathological changes in pancreas tissue are more significant than that in small-intestine mucosa in mice

关 键 词：发酵支原体 梨支原体 电镜 胰腺 小肠黏膜 

分 类 号：R375[医药卫生—病原生物学]