3株人源猪链球菌2型湖南株cps2J、gdh、ef基因进化分析  

Phylogenetic analysis of cps2J,gdh and ef genes of 3 human-derived Streptococcus suis serotype 2 strains isolated in Hunan Province

作　　者：欧新华[1] 张如胜[1] 苏良[1] 杨柳青[1] 肖姗[1] 姚栋[1] 宋克云[1] 孙边成[1] 

机构地区：[1]长沙市疾病预防控制中心,长沙410006

出　　处：《中国人兽共患病学报》2013年第4期369-374,共6页Chinese Journal of Zoonoses

基　　金：长沙市科技计划项目(K0902167-31)~~

摘　　要：目的对3株人源猪链球菌2型(S.suis 2)湖南株(HNCS201001、HNCS201101、HNCS201102)cps2J、gdh、ef基因进行序列测定和进化分析,了解其变异和进化状况,为人感染S.suis疫情的防控提供数据。方法设计S.suis 2 cps2J、gdh、ef基因PCR引物,对HNCS201001、HNCS201101、HNCS201102株进行PCR扩增和序列测定,测序结果利用Lasergene和Mega5软件进行分析。结果 cps2J、gdh、ef基因包含的完整开放阅读框(ORF)分别为999bp、1 347bp、2 532bp,分别编码333、448、843个氨基酸。在线BLAST同源性分析表明,HNCS201001、HNCS201101、HNCS201102株属于S.suis 2,3菌株cps2J、gdh、ef基因组序列之间的核苷酸同源性均为100%;进化树显示,HNCS201001、HNCS201101、HNCS201102株与国内外猪源和人源S.suis 2菌株属于同一分支,同源性比较显示3株S.suis 2湖南分离株cps2J、gdh、ef基因与其他国内外S.suis株核苷酸序列同源性分别为99.8%～100%、96.4%～100%和99.7%～100%,氨基酸同源性分别为98.5%～100%、98.7%～100%和99.5%～100%。结论 3株人源S.suis 2湖南株cps2J、gdh、ef基因序列与国内外S.suis 2菌株相应基因序列同源性高,变异程度小。In order to explore the variation and evolutionary status of Streptococcus suis serotype 2 （S. suis 2）isolated in Hunan Province, gene sequences and evolution relationship of cps2J, gdh and ef genes of HNCS201001, HNCS201101, and HNCS201102 of human derived S. suis 2 strains were analyzed. PCR primers of cps2J, gdh and ef genes of S. suis 2 were de- signed, and the genes of HNCS201001, HNCS201101, and HNCS201102 were amplified and sequenced. Then, the nucleotide sequences and the deduced amino acid sequences were analyzed with the Lasergene and Mega 5 software. Analysis of the nucle- otide sequences of the cps2J, gdh and ef genes revealed that the length of open reading frames （ORF） were 999 bp, 1 347 bp, and 2 532 bp, coding for 333, 448 and 843 amino acid, respectively. Sequence similarity searches by using the BLAST network service showed HNCS201001, HNCS201101 and HNCS201102 strains belonged to S. suis 2 group, and the nucleotide homolo- gy of cps2J, gdh and ef genes among the 3 strains were all 100% ; phylogenetic analysis revealed that the 3 strains in this study and the S. suis 2 strains derived from pigs and human isolated in China's Mainland and abroad belonged to the same branch. The cps2J, gdh and ef genes of the 3 strains of S. suis 2 isolated in Hunan Province had high homology with other S. suis strains isolated in China's Mainland and abroad （99.8^-100~ for cps2J gene, 96.4^-100~ for gdh gene, and 99.7^-100~ for ef gene at the nucleotide level; 98.5^-100~ for cps2J gene, 98.7%-100% for gdh gene, and 99.5%-100% for ef gene at thea- mino acid level, respectively）. It＇s suggested that the cps2J, gdh and ef genes of the 3 human-derived S. suis 2 strains isolated in Hunan Province have high homology with other S. suis 2 strains isolated in China's Mainland and abroad, and have a low de- gree of variation in the gene sequences.

关 键 词：猪链球菌2型 cps2J基因 gdh基因 ef基因 进化分析 

分 类 号：R378.1[医药卫生—病原生物学]