外源性腺苷对心肌线粒体氧耗反应时间的影响  

The effects of exogenous adenosine on the mean response time of myochardial mitochondrial oxygen consumption

作　　者：田新强[1] 

机构地区：[1]大同医学高等专科学校,大同037008

出　　处：《华北煤炭医学院学报》2000年第3期241-243,共3页Journal of North China Coal Medical College

基　　金：1997年山西省出国留学基金资助项目

摘　　要：探讨外源性腺苷对心肌线粒体平均氧耗反应时间的影响。方法　采用 Van Beek等建立的兔离体心脏灌流方法 ,测定心肌线粒体平均氧耗反应时间 ( tmito)。结果　腺苷组 tmito随心率变化的增加而明显延长。当心率变化从 6 0 /min,分别增加至 70 ,80 ,1 0 0 ,1 2 0 /min时 ,测得 tmito分别为 5 .6 ,6 .3 ,8.0 ,1 1 .3 s( P<0 .0 5 )。无腺苷组分别为 6 .7,8.1 ,8.4,8.6 s( P>0 .0 5 ) ,tmito未出现随心率增加而延长的现象 ,平均值为 8.0 s。结论　腺苷可能是通过刺激葡萄糖酵解 ,进而增加线粒体外 ATP的合成 ,而延缓线粒体氧化磷酸化过程 ,以达到对心肌分解Purpose To investigate the effect of exogenous adenosine on the response time of myocardial mitochondrial oxygen consumption (t mito ).[Methods The method developed by Van Beek et al was adapted to determined t mito .Results We found that t mito was increased significantly with heart rate steps (P<0.05),the t mito was 5.6,6.3,8.0 and 11.3s when heart rate was 60 min -1 to 70,80,100 and 120 min -1 respectively with exogenous adenosine(10 -5 mol/L) in perfusate.But in group without adenosine in perfusate the t mito was 6.7,8.1,8.4 and 8.6s respectively with same heart rate steps as before.There was no significant change in t mito (P>0.05).Conclusions Adenosine delayed the adapting speed of myocardial mitochondrial oxidative phosphorylation to demand with heart rate steps.

关 键 词：心肌 线粒体 外怀腺苷 家兔 氧化磷酸化 

分 类 号：R331.3[医药卫生—人体生理学]