应用CBA法检测HLA单倍体相合非清髓造血干细胞移植中Th1/Th2/Th17细胞因子的变化  

Changes of Th1/Th2/Th17 in Patients Recieved Non-myeloablative Haploidentical Hematopoietic Stem Cell Transplantation Detected by Flow Cytometric Bead Array

作　　者：赵月莹[1] 康严[1] 孙琪云[1] 董征[1] 刘铁强[1] 刘广贤[1] 郭梅[1] 艾辉胜[1] 

机构地区：[1]军事医学科学院附属医院血液科,北京100071

出　　处：《中国实验血液学杂志》2013年第2期431-435,共5页Journal of Experimental Hematology

摘　　要：本研究探讨非清髓异基因外周造血干细胞单倍体相合移植(NAHSCT)中,Th1/Th2/Th17细胞因子的变化。用流式微珠阵列法(flow cytometric bead array,CBA)检测18例allo-NAHSCT患者移植前、后不同时间点,血清细胞因子IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ、IL-17水平的变化。结果表明,移植后所有的细胞因子均明显改变,其表达较移植前增加;IL-2、IL-4、IL-17发生变化的时间较早,移植后1周其表达开始明显上调;IL-6、IL-10、TNF-α移植后2周起显著改变,与移植前相比血清浓度明显提升;INF-γ变化较晚,移植后4周表达开始增高;所有的细胞因子都在移后4周内呈持继上升趋势,至第4周达峰值。结论:非清髓异基因外周血单倍体相合移植后患者血清细胞因子在早期均发生显著性提升;IL-2,IL-4,IL-17变化较早,IFN-γ变化相对迟缓,至第4周为其峰值;Th1/Th2/Th17细胞因子移植早期的变化规律可能与患者的综合情况相关。This study was purposed to investigate the changes of Th1/Th2/Th17 in patients received non- myeloablastic haploidectical hematopoietic stem cell transplantation （NAHSCT）. The levels of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ, as well as IL-17 level were determined by flow cytometric bead array （CBA） in samples from 18 patients undewent allo-peripheral NAHSCT at different time points before and after transplantation. The results showed that all cytokines changed obviously after transplantation, and their serum levels were higher than that before transplantation. The expression levels of 1L-2, IL-4 and IL-17 changed early, and their obviously up-regulation was found after transplantation. The expression levels of IL-6, IL-10 and TNF- α changed significantly, and were high as compared with that before transplantation. The change of INF-γ serum level was observed late, its rising occurred at week 4 after transplantation. The expression of all cytokines kept increasing during 4 weeks after transplantation and peaked at week 4. It is concluded that the serum levels of all cytokines from the patients after NAHSCT increased significantly, in which the levels of IL-2, IL-4 and IL-17 increased early, but the level of INF-γ changed late. The detection of cytokines is helpful for deep understanding the pathophysiologic mechanism of transplant-related complications.

关 键 词：非清髓异基因单倍体相合干细胞移植 血清细胞因子 流式微珠阵列 

分 类 号：R457.7[医药卫生—治疗学] R733.7[医药卫生—临床医学]