多供体造血干细胞移植术后的嵌合率定量检测  

Quantitative Monitoring of Multi-donor Chimerism after Multi-donor Allogeneic Hematopoietic Stem Cell Transplantation

作　　者：冯宇锋[1] 张翔[1] 陈广华[1] 徐杨[1] 龚斐然[1] 朱子玲[1] 戴丽君[1] 宋铁梅[1] 周佳子[1] 唐晓文[1] 常惠荣[1] 缪竞诚[2] 吴德沛[1] 

机构地区：[1]苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,江苏苏州215006 [2]苏州大学医学部基础医学与生物科学学院,江苏苏州215124

出　　处：《中国实验血液学杂志》2013年第2期436-440,共5页Journal of Experimental Hematology

基　　金：江苏高校优势学科建设工程资助项目(2010);国家高技术研究发展计划(863计划)课题(编号2011AA020105);江苏省临床医学中心项目(编号ZX201102)

摘　　要：本研究旨在建立检测多供体造血干细胞移植术后供体细胞嵌合率(DD-CHM%)的计算模型并简化计算方法。以接受异基因造血干细胞移植的血液系统疾病患者为研究对象,采用STR-PCR结合毛细管电泳的方法,检测移植术后供体细胞嵌合率。结果表明:在混合嵌合状态下,同一个体来源的等位基因的双峰面积和高度是近似的,可以互相替代;对构建的计算模型中各等位基因的峰面积进行赋值,得到的嵌合率准确值和近似值在可接受的计量系统自身误差范围内(5%-20%)是基本没有差异的。结论:在混合嵌合状态下,来自于同一个体的等位基因在STR位点上的非共享峰是可以替代共享峰面积,此设想可以用作于计算多供体造血干细胞移植术后嵌合率的检测;在计算位点选择上,受体位点的选择比供体位点选择更为重要,能更加有效的简化计算步骤,并能更准确的得到供体细胞嵌合率的结果。This study was aimed to establish a model for detecting the donor chimerism rate following the multi-donor hernatopoietic stem cell transplantations, and simplify its calculation method. Patients with hematologic disease receiving allogeneic hematopoietic stem cell transplantation including single -donor and multi-donor were selected in this study and the donor cell chimerism rates were detected,using STR-PCR combined with capillary electrophoresis. The results indicated that the peaks of the sister alleles coming from the same individual were confn＇rned to have the approximate areas and can be replaced each other in the situation of mixed chimerism. In the calculation model, the value between reference chimedsm and approximate chimerism have no significant difference using the hypothetical peak areas, and the result was confirmedto be accepted basing on typical measurement error between sister alleles（5% -20% ）. It is concluded that the areas of share peaks can be replaced by non-share peaks and this conclusion can be used to calculate the double-donor CHM （DD-CHM） （%）. Compared to the D alleles, R alleles show more strategic importance because it can lead to more accurate result and allowed simplifying the arithmetic calculations for DD-CHM（ % ）.

关 键 词：异基因造血干细胞移植 多供体 嵌合率 共享峰 

分 类 号：R457.7[医药卫生—治疗学]