活血、破血药对动脉粥样硬化大鼠PCNA蛋白、VEGFmRNA、VEGFR-2mRNA表达的影响  

Influence of blood-activating and blood-breaking medicines on the PCNA protein and VEGF mRNA,VEGFR-2 mRNA expression in the rat model with atherosclerosis

作　　者：谢海波[1] 罗尧岳[2] 莫新民[2] 吴亦之[1] 卢青[1] 徐豫湘[1] 李武[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007

出　　处：《湖南中医药大学学报》2013年第3期23-26,共4页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(10JJ6029);湖南省教育厅优秀青年项目(09B076)

摘　　要：目的探讨活血药(当归、川芎)、破血药(三棱、莪术)对动脉粥样硬化(AS)的大鼠主动脉增殖细胞核抗原(PCNA)蛋白及血管内皮生长因子(VEGFmRNA)、血管内皮生长因子受体(VEGFR-AS)表达的影响。方法将60只雄性Wistar大鼠随机分为5组(空白组、模型组、他汀组、活血组、破血组),先采用高脂喂饲AS大鼠,造模成功后,灌胃给药,连续4周。实验结束后,用免疫组化染色法检测主动脉内膜PCNA蛋白表达;用原位杂交法检测主动脉内膜VEGFmRNA、VEGFR-2mRNA的基因表达。结果破血组PCNA阳性细胞数低于活血组(P<0.05);模型组VEGFmRNA、VEGFR-2mRNA平均灰度值均高于空白组、他汀组、活血组和破血组(P<0.05);活血组、破血组组间比较无差异。结论当归、川芎与三棱、莪术均能抑制PCNA、VEGFmRNA及VEGFR-2mRNA的表达,从而起到抑制细胞增殖的作用。在抑制PCNA表达方面,破血药三棱、莪术优于活血药当归、川芎。Objective To discuss the influence of blood-activating（angelica sinensis and ligus- ticum wallichii） and blood-breaking medicines（rhizoma sparganii and curcuma zedoary） on the PCNA protein and VEGFmRNA, VEGFR-2 mRNA expressions in the aortic tunica intima of rats with atherosclerosis （AS）. Methods 60 male Wistar rats were randomly divided into 5 groups （including blank group, model group, statins group, blood-activating medicine group and blood-break- ing medicine group） and AS rat model was established by feeding high -fat forage. These medicines were given by intragastric administration for 4 weeks. After treatment, PCNA pro- tein expression level was detected with immunohistochemical staining method and the VEGF mRNA, VEGFR-2 mRNA gene expression levels were tested with hybridization in situ of the aortic tunica intima. Results The positive cell amount of PCNA in the blood-breaking medicine group was lowerthan that in the blood-activating medicine group 2 mRNA average gray values in the model group （P〈0.05）. The VEGF mRNA and VEGFR- were higher than those in the blank group, statins group, blood-activating medicine group and blood-breaking medicine group, while there were no significant differences between blood-activating medicine group and blood-break- medicine group. Conclusion Angelica sinensis, Ligusticum wallichii, rhizoma sparganiiand curcuma zedoary can inhibit the expression of PCNA protein, VEGF mRNA and VEGFR- 2 mRNA, and then inhibit the cell proliferation. The inhibiting effects on the PCNA expression of blood-breaking medicines （rhizoma sparganii and curcuma zedoary） are better than those of blood- activating medicines（Angelica sinensis and Ligusticum wallichii）.

关 键 词：当归 川芎 三棱 莪术 动脉粥样硬化 大鼠 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]