海马齿SpSCL1基因启动子克隆及序列分析  

Cloning and sequence analysis of SpSCL1 promoter from Sesuvium portulacastrum

作　　者：刘习文[1] 畅文军[2] 朱家红[2] 张治礼[2,3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]海南省农业科学院,海南海口571000

出　　处：《广东农业科学》2013年第4期124-127,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31000313);中央级公益性科研院所业务费专项资金(ITBB110201)

摘　　要：GRAS蛋白是植物特有的一类蛋白家族,在植物的生长发育方面具有重要作用。根据前期分离的海马齿SpSCL1基因的5'端序列,设计特异引物进行Genome Walking,从海马齿基因组DNA中克隆了SpSCL1基因上游的1 254 bp片段。序列分析表明,该序列包含681 bp的启动子调控序列及573 bp的基因编码序列,启动子调控序中除含有典型的真核生物核心启动子区域(-60^-10bp)外,还含有多个TATA-box、CAAT-box等启动子元件以及多种与脱水、耐盐和光响应相关的顺式作用元件。结果预示海马齿SpSCL1可能在抗旱、耐盐以及光敏色素信号转导途径中发挥重要作用。The GRAS proteins are a recently identified family of plant-specific proteins, which play a crucial role in diverse plant growth and development. Specific primers was designed according to the 5 legion of SpSCL1 gene from Sesuvium portulacastrum, the 1 254 bp 5regulatory sequenee of SpSCL1 was cloned from shoot genomie DNA of Sesuvium portulacastrum by Genome Walking method. Sequence analysis showed the sequence contains 681 bp of the promoter regulation sequence and the gene coding region sequence of 573 bp, the promoter regulation sequence consisted of a typical core promoter region of eukaryotic （-60-10 bp）, several promoter element such as TATA-box and CAAT-box, as well as many cis-regulatory elements related to salt, dehydration and stress responses, there are also some eis-regulatory elements related to light responses. It suggested that SpSCL1 gene might play an important role in the salt tolerance, drought tolerance, phytochrome signal transduction pathways.

关 键 词：海马齿 SpSCL1启动子 顺式调控元件 耐盐 抗旱 光敏色素信号转导 

分 类 号：Q781[生物学—分子生物学]