贵州省五烟区烟草CMV、PVY带毒率检测  

The detection on CMV、PVY of tobacco in five different tobacco regions of Guizhou

作　　者：张福莉[1] 刘童童[1] 李创创[1] 杨茂发[1] 杨洪[1] 

机构地区：[1]贵州大学昆虫研究所/贵州山地农业病虫害重点实验室,贵州贵阳550025

出　　处：《广东农业科学》2013年第4期131-133,共3页Guangdong Agricultural Sciences

基　　金：中国烟草总公司贵州省公司科技项目(201022)

摘　　要：采用间接酶联免疫吸附分析法对贵州省5个主要烟草种植区的田间烟草进行了黄瓜花叶病毒病和马铃薯Y病毒病的检测,并采用一步RT-PCR法验证试验的准确性。结果表明:思南、凤冈、德江、正安和清镇5个烟区烟草的CMV带毒率分别为66%、67%、54%、59%和40%,PVY带毒率分别为54%、56%、37%、49%和36%,复合侵染率分别为32%、23%、27%、23%和7%。Indirect enzyme linked immunity absorb assay was used to detect Cucumber mosaic virus disease and Potato virus Y of tobacco in five main tobacco planting areas of Guizhou, and one step RT-PCR method was used to verify the accuracy of the experiment. The results showed that the CMV infectivity of tobacco in Sinan, Fenggang, Dejiang, Zheng＇an and Qingzhen were 66%, 67%, 54%, 59% and 40% respectively, the PVY infectivity of tobacco in five tobacco regions were 54%, 56%, 37%, 49% and 36% respectively, and the mixed infection rate were 32%, 23%, 27%, 23% and 7% respectively.

关 键 词：酶联免疫吸附分析法 黄瓜花叶病毒 马铃薯Y病毒 烟草 贵州 

分 类 号：S432.41[农业科学—植物病理学]