花鳗鲡病原菌弗氏柠檬酸杆菌的鉴定  

Identification of Pathogenic Citrobacter freundii Isolated from Anguilla marmorata

作　　者：杨方园[1] 关瑞章[1,2,3] 李忠琴[1,2,3] 于海振[1] 李秋云[1] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]农业部东海海水健康养殖重点实验室,福建厦门361021 [3]集美大学鳗鲡现代产业技术教育部工程研究中心,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2013年第2期81-87,共7页Journal of Jimei University：Natural Science

基　　金：农业部公益性行业(农业)科研专项(nyhyzx07-043);福建省科技计划重点项目(2010N0022;2009N0046);厦门市科技计划重点项目(3502Z20103023)

摘　　要：首次报道了从养殖花鳗鲡(Anguilla marmorata)肝脏中分离得到优势菌株AMRB6,经人工腹腔注射感染健康花鳗鲡试验,结果证实该菌有致病作用,其半数致死量LD50为3.2×107CFU/mL.结合菌体形态特征、常规生理生化鉴定和Biolog自动微生物鉴定系统鉴定,以及16S rRNA序列扩增结果,表明该菌为弗氏柠檬酸杆菌(Citrobacter freundii).30种药物药敏试验结果表明,该菌株对左氧氟沙星、诺氟沙星、庆大霉素、卡那霉素等14种药物高度敏感.The paper was the first one reported that a pathogenic bacterium, named AMRB6, was isolated from the liver of diseased AnguiUa marmorata. The strain was proven to be a pathogen of eel by intraperitoneal injection with bacteria suspension, and the LD50 was 3.2×10^7CFU/mL. Based on the morphological characters of bacteria, traditional physiological and biochemical identification and Biolog identification system, and analysis of 16S rRNA gene, the strain was identified to be Citrobacterfreundii. In antibiotic sensitivity test to 30 antimicrobial agents, the stain had high sensitivity to 14 agents including levofloxacin, norfloxacin, gentamicin, and kanamycin.

关 键 词：花鳗鲡 弗氏柠檬酸杆菌 药敏试验 病原菌 

分 类 号：S941[农业科学—水产养殖]