黄芪多糖对新城疫弱毒疫苗免疫雏鸡血凝抑制抗体水平的影响  

Effects of Astragalus Polysaccharides on HI Antibody Titer after Vaccination of Low Toxicity Live Vaccine of the Newcastle Disease in Chickens

作　　者：郝桂英[1] 钟元良[2] 

机构地区：[1]西昌学院动物科学学院,四川西昌615013 [2]隆昌县畜牧局动物卫生监督所,四川隆昌642150

出　　处：《西昌学院学报（自然科学版）》2013年第1期14-16,共3页Journal of Xichang University(Natural Science Edition)

摘　　要：40只1日龄健康雏鸡随机分成4组,Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ为黄芪多糖试验组,每组10只,置于相同的条件下饲养,在第5日龄时对Ⅱ-Ⅳ组每只鸡分别颈部皮下注射0.1mg、0.3mg和0.5mg黄芪多糖注射液,对照组注射生理盐水,每天1次,连续3d,并于第7和21日龄时采用滴鼻、点眼的方式给各组鸡接种新城疫Lasota弱毒疫苗,用β-微量法检测血清中血凝抑制抗体效价的动态变化。结果表明,黄芪多糖对鸡新城疫免疫效果有明显的促进作用,与对照组(Ⅰ组)差异显著,其中以黄芪多糖0.5 mg/只时为最佳使用剂量。由此可见,黄芪多糖在一定剂量范围内可明显增强对鸡新城疫免疫的效果。40 healthy one-day-old chickens were selected in this experiment, which were randomly divided into four groups, so each group were 10, the group I was control group, the three experimental groups （II, III and IV group） were subcutaneous injected with Astraglus polysacchride. They were fed in the same conditions. In the fifth day, each chicken of II, III and IV group was given 0.1mg, 0.3mg and 0.5mg of APS by subcutaneous injection, respectively. Physiological saline was injected in control group, which was injected once a day for three successive days. Lasota vaccine of ND was given by eye and nasal droping at 7-day-old and 21-day-old, respectively. The dynamic variation of serum HI antibody was determined by [3 -micromethod. The results showed that Aps played a promoting role in the immune effects of ND. There were distinct differences between experiment groups and the control group. While the best dose of Astragalus polysaccharides was 0.5 mg per each. It could conclude that a certain dose of APS can significantly enhance the effect of Newcastle disease immunization.

关 键 词：黄芪多糖 新城疫 血凝抑制抗体效价 

分 类 号：S858.31[农业科学—临床兽医学]