复方红花微乳中利多卡因含量的测定  

Determination of Lidocaine Content in Compound Carthamus tinctorious Microemulsion by UPLC

作　　者：杨李旺[1] 季新燕[1] 冯前进[1] 常瑞华[1] 梁红娟[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2013年第1期26-28,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：山西省科技厅条件平台项目(2011091014);太原市科技局产业技术创新与成果转化平台建设专项(2012-3-PT-F-125);山西中医学院校级课题项目(2011JC007)

摘　　要：目的:建立复方红花微乳中利多卡因含量的UPLC测定方法。方法:采用高效液相色谱法,色谱柱:Diamonsil钻石C1(85μm,250 mm×4.6 mm);流动相:甲醇-0.4%的磷酸水溶液(40∶60,三乙胺调至pH=3.30);检测波长:200 nm;柱温:室温;流速:0.8 mL/min。结果:利多卡因在0.01 mg/mL～0.5 mg/mL范围内线性关系良好(r=0.999 2),平均回收率为98.83%,RSD=1.94%(n=6)。结论:该法测定复方红花微乳中利多卡因含量准确可靠,简单易行,重现性好,可用于复方红花微乳中利多卡因含量的质量控制。Objective：To establish UPLC method for the determination of lidocaine content in compound Carthamus tinctorius microemulsion.Method：The determination was performed on Diamonsil C18 column（5 μm,250 mm×4.6 mm）with methanol-H2O（40∶60）as mobile phase.Flow rate was at 0.8 mL/min and detecting wave length was at 200 nm.Results：The linear range of lidocaine was at 0.01 mg/m～0.5 mg/mL（r=0.999 2）.Average recovery rate was 98.83%（RSD=1.94%）.Conclusion：The method was simple,accurate and reproducible,and could be used for determination of lidocaine content in compound Carthamus tinctorius microemulsion.

关 键 词：UPLC 复方红花微乳 利多卡因 

分 类 号：R285[医药卫生—中药学]