过氧化物酶在多孔磁性微球修饰电极上的直接电化学行为研究  

Study of the direct electrochemistry behavior of peroxidase at the porous magnetic microspheres modified electrode

作　　者：谭向恩[1] 赖国松[1] 韩德艳[1] 张海丽[1] 

机构地区：[1]湖北师范学院化学与环境工程学院,湖北黄石435002

出　　处：《湖北师范学院学报（自然科学版）》2013年第1期65-68,共4页Journal of Hubei Normal University(Natural Science)

基　　金：国家自然科学基金(21075030);湖北省教育厅重点项目(D2010250)

摘　　要：采用直接滴涂法制备多孔磁性微球/过氧化物酶(PMMS/POD)修饰的碳糊电极。运用电化学阻抗法、循环伏安法和稳态安培法对该修饰电极进行表征和研究。系统地考察了底液pH值,扫描速率对修饰电极影响,找出最佳实验条件。计算出扩散系数D为5.03×10-10cm2.s-1,表征酶活性的米氏常数为0.069 m mol.L-1.在优化条件下,该H2O2传感器的响应时间小于10s,考察了该修饰电极对H2O2稳态安培响应,在0.1m mol.L-1～0.8 m mol.L-1浓度范围内,稳态安培电流与H2O2浓度成较好线性关系,检测限为0.03 m mol.L-1(S/N=3).A novel porous -magnetic chitosan microspheres （PMMS） was prepared. Peroxidase （POD） was successfully im- mobilized on the surface of PMMS modified carbon paste electrode （CPE） with the cross - linking of glutaraldehyde. The modi- fied electrode were characterized by electrochemical impedance spectroscopy （ EIS）, cyclic voltammetry （ CV ） and steady - state amperometry. The effect of the experimental variables such as solution pH, scan rate were investigated, This biosensor had a fast response of H2O2 ＂less than 10s and excellent linear relationships were obtained in the concentration range of 0. 1 mmol - L-1 -0.8mmol . L-1 with the detection hmlt of 0.03 mmol . L-1. （ S/N = 3 ）. Moreover, the apparent Michaelis - Menten constant was estimated to be 0.069 mmol .L-1.

关 键 词：生物传感器 过氧化物酶 多孔磁性微球 化学修饰电极 直接电化学 

分 类 号：O657.1[理学—分析化学]