体外测定血管紧张素转化酶抑制活性中4种酶失活的方法  

Comparative analysis of four different enzyme inactivation methods for determination of ACE-inhibitory activity in vitro

作　　者：陈永福[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2013年第4期7-9,31,共4页China Dairy Industry

基　　金：国家自然科学基金(31101315);内蒙古自然科学基金(2010M1301)

摘　　要：分析了不同浓度的盐酸溶液、有机溶剂(乙腈、丙酮、甲醇)、不同浓度的EDTA溶液和不同温度下加热等4种酶失活方法对ACE抑制活性体外测定结果的影响。结果表明:温度为85℃加热15 min和添加浓度为0.100 mol/L的EDTA溶液方法不仅使ACE失活,而且不影响反应液中马尿酸质量浓度,从而保证了方法的稳定性和重现性,故二者均为切实可行的ACE失活方法。In this paper, we performed a comparative analysis of four different enzyme inactivation methods, namely by adding hydrochloric acid, organic solvents （acetonitrile, acetone and methanol） and EDTA, and using heat for determination ACE-inhibitory activity in vitro. The results showed that the method by using heat （85℃, 15 min ） and adding EDTA solution （containing 0.100 mol/L ofEDTA ） not only inactivated ACE, but also had no effect on the hippuric acid content in the reaction mixture, so as to ensure the stability and the reproducibility of the method for ACE-inhibitory determination. To sum up, these two methods are preferable ACE inactivation methods.

关 键 词：血管紧张素转化酶 失活 体外 

分 类 号：Q814[生物学—生物工程]