利拉鲁肽对人脐静脉内皮细胞中核转录因子κB和单核细胞趋化蛋白1表达的影响  

The effect of liraglutide on the expression of nuclear factorkappa B and monocyte chemoattractant protein-1 in human umbilical vein endothelial cells

作　　者：张艳[1] 李伟[2] 

机构地区：[1]徐州医学院,江苏徐州221002 [2]徐州医学院附属医院内分泌科,江苏徐州221002

出　　处：《中国现代医学杂志》2013年第6期29-33,共5页China Journal of Modern Medicine

基　　金：徐州市科技局基金(No:XM08C072)

摘　　要：目的观察胰高糖素样肽-1(GLP-1)类似物利拉鲁肽(LRL)对高糖及脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)核转录因子-κB(NF-κB)和单核细胞趋化蛋白1(MCP-1)表达的影响。方法体外培养HUVECs,细胞传至第3代,随机分为7组:正常对照组(C组),高糖组(HG组),LPS组,高糖+LPS组(HGS组),高糖+LRL组(HGL组),LPS+LRL组(LPSL组)和高糖+LPS+LRL组(HGSL组)。CCK-8法检测LRL干预前后HUVECs的增殖情况,RT-PCR和ELISA分别检测NF-κB、MCP-1 mRNA和蛋白的表达情况。结果与C组比较,HG组、HGS组、LPS组HUVECs的生长受到明显的抑制(P<0.05),LRL干预后各组细胞的生长抑制情况得到减轻(P<0.05)。HG组、LPS组、HGS组HUVECs中NF-κB、MCP-1的mRNA和蛋白水平较C组升高(P<0.05);LRL干预后各组mRNA和蛋白水平降低(P<0.05)。结论高糖和LPS抑制HUVECs增殖,促进NF-κB、MCP-1表达增加,LRL能有效降低炎症因子的表达,改善内皮功能紊乱,有助于延缓糖尿病动脉粥样硬化的进程。【Objective】To investigate effects of liraglutide（LRL） on the expressions of nuclear factor-kappaB（NF-κB） and monocyte chemoattractant protein-1（MCP-1） mRNA and protein in human umbilical vein endothelial cells（HUVECs） cultured under high glucose and LPS environment,and its mechanisms of anti-diabetic artherosclerosis.【Methods】HUVECs were cultured in vitro and at the 3rd generation were divided into seven groups： normal control group（group C）,high glucose group（group HG）,LPS group（group LPS）,HG plus versus concentration of LPS group（group HGS）,group HG plus LRL（group HGL）,group LPS plus LRL（group LPSL）,group HGS plus LRL（group HGSL）.CCK-8 used to detect the proliferation of HUVECs in different groups before and after LRL intervention;mRNA expressions of NF-Κb,MCP-1 were determined by RT-PCR;the protein of NF-κB,MCP-1 were determined by ELISA.【Results】The proliferation of HUVECs in HG,LPS and HGS groups were lower than C group（P 0.05）,after LRL treatment they became higher than the latter（P 0.05）.The mRNA and protein expression levels of NF-κB and MCP-1 in HUVECs of HG,HGS and LPS groups were higher than that of C group（P 0.05）.The mRNA and protein expression levels of NF-κB and MCP-1 in cells treated with LRL were lower than before treatment（P 0.05）.【Conclusion】LRL plays a key role in delaying atherosclerosis by decreasing the expression of NF-κB and MCP-1.

关 键 词：糖尿病 内皮细胞 脂多糖 NF-κB MCP-1 利拉鲁肽 

分 类 号：R587.2[医药卫生—内分泌]