黄精种子破眠技术研究  

A Study On Technology For Breaking Dormancy of Polygonatum sibiricum Red. Seeds

作　　者：朱伍凤[1] 王剑龙[1] 常辉[1] 刘峰 张跃进[1] 张小燕[3] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]咸阳步长制药有限公司,陕西西安710075 [3]西北农林科技大学农学院,陕西杨陵712100

出　　处：《种子》2013年第4期13-16,19,共5页Seed

基　　金：黄精GAP基地建设项目(编号:2008 BC-01);秦巴山区重要林药泛素化筛选与规范化栽培关键技术研究(编号:201204603)

摘　　要：以黄精种子为材料,研究了不同光照、沙藏温度、温水及外源生长调节物质浸种对黄精种子萌发的影响,为进一步开展黄精有性繁殖提供技术支持。结果表明:在0℃低温沙藏120 d的条件下,最有利种子的萌发;黑暗条件下种子萌发率比光照条件下萌发率高37.78%,其差异显著,说明黑暗更有利于黄精种子的萌发;40℃温水浸种24～30 h,可有效促进黄精种子的萌发,发芽率达到93.33%。适宜浓度的GA3、6-BA、水杨酸和壳聚糖均能促进黄精种子的萌发,显著缩短发芽时间,其发芽率、发芽势、发芽指数和活力指数等指标均明显高于对照。其中浓度为150 mg/L的GA3浸种24 h,发芽率、发芽势、发芽指数及活力指数均最高,分别为91.11%、62.22%、0.87和5.16;100 mg/L的6-BA除了可以促进种子萌发,还可以打破黄精种子萌发过程中芽的休眠。In order to provide technical support for sexual propagation of Polygonatum sibiricum Red ,we stud- ied the effects of illumination, sand storage temperature, lukewarm water and exogenous growth regulating sub- stances on the seed germination of Polygonatum sibiricum. The results showed that ： Storing at 0℃ low-temper- ature sand under the condition of 120 days was favorite for seed germination;Seed germination rate under dark conditions was 37.78% which was higher than that under illumination of high germination rate, indicating that dark was beneficial for the germination of Polygonatum sibiricum seeds ;After soaked 18 -24 hours in 35 -40 ~C lukewarm waters ,the germination rate of Polygonatum sibiricum seeds reached to 93.33%. Suitable concen- tration of GA3, 6-BA, salicylic acid and chitosan increased the germination of Polygonatum sibiricum seeds sig- nificantly, and the germination time shortened remarkably. The concentration for GA3 （150 mg/L） seed soaking 24 hours had the best effect, germination rate, germination potential, germination index and vigor index were up to 91.11% ,62.22% ,0.87 and 5.16 ,respectively;6-BA （ 100 mg/L） still can break bud dormancy in ad- dition to promote seed germination.

关 键 词：黄精 种子 破眠技术 

分 类 号：S567.2[农业科学—中草药栽培]