灰毡毛忍冬对小鼠免疫调节及肝损伤保护作用研究  

The Immunomodulatory and the Protection of Experimental Liver Injury of Lonicera macranthoides Hand.-Mazz on Mice

作　　者：薛强[1] 刘杨[1] 李燕君[1] 李莉[1] 王森弘[1] 徐晓玉[1] 

机构地区：[1]西南大学药学院中医药学院,西南大学中药研究所,重庆市药效评价工程技术研究中心,重庆市银花工程技术研究中心,国家中医药管理局中药药理学重点建设学科,重庆400716

出　　处：《中国药学杂志》2013年第8期601-606,共6页Chinese Pharmaceutical Journal

基　　金：国家科技支撑计划课题(2006BAC01A16);科技部富民强县专项行动计划项目(国科发农[2008]471号)

摘　　要：目的研究灰毡毛忍冬花蕾对小鼠免疫调节和肝损伤保护作用。方法采用环磷酰胺致小鼠免疫功能低下模型结合腹腔巨噬细胞和脾淋巴细胞体外增殖实验,评价其免疫调节作用;分别用四氯化碳和酒精诱导小鼠肝损伤模型,评价其对肝损伤的保护作用。结果灰毡毛忍冬1.6、3.2和6.4 g.kg-1均能显著提高免疫低下小鼠胸腺指数、脾指数、巨噬细胞碳廓清与吞噬指数以及腹腔巨噬细胞和脾淋巴细胞增殖能力(P<0.01),3.2和6.4 g.kg-1均能显著降低四氯化碳和酒精诱导肝损伤小鼠血清中丙氨酸氨基转移酶、天冬氨酸氨基转移酶水平和肝组织中丙二醛水平(P<0.01),显著升高肝组织中超氧化物歧化酶水平(P<0.01),明显减轻肝组织的病理学损害。结论灰毡毛忍冬能显著提高环磷酰胺诱导的免疫低下小鼠免疫功能,对四氯化碳和酒精导致的小鼠肝损伤有较好的保护作用。OBJECTIVE To study the immunomodulatory and the protection of experimental liver injury of Lonicera macranthoides Hand. -Mazz （LHM） which is the main source of Lonicera confusa DC. METHODS Immunocompromised mice model induced by intraperitoneal injection of cyclophosphamide and in vitro proliferation experiment of its peritoneal macrophages and spleen lymphocytes were used to evaluate the immunomdulatory effect of LHM. The inhibition of LHM on hepatic injury induced by CC14 or alcohol on mice was measured. RESULTS The doses of 1.6, 3.2 and 6.4 g kg- 1 of LHM could significantly increased the thymus index, the spleen index ,the carbon dissection index and the macrophage phagocytic index of mice （P 〈 0.01 ）, and obviously raised the proliferation ability of peritoneal macrophages and transformation ability of splenic lymphocytes （P 〈 0. 01 ）. The doses of 3.2 and 6. 4 g kg- 1 of LHM could significantly lower the incremental ALT, AST and MDA level（ P 〈 0. 01 ）, raise the low SOD level in liver （P 〈 0. 01 ） of hepatic injury mice induced by CC14 or alcohol, and obviously lighten the pathological damage of hepatic tissue. CONCLUSION The LHM could significantly promote the proliferation and phagocytosis function of immunocytes on immunocompromised mice. Moreover, it has good protection effect on hepatic injury of mice which is induced by CCl4 or alcohol.

关 键 词：灰毡毛忍冬 免疫调节 肝损伤 

分 类 号：R965[医药卫生—药理学]