肌苷分子印迹聚合物的合成及其吸附性能研究  

Synthesis and adsorption properties of inosine molecularly imprinted polymers

作　　者：王美华[1] 黄丽英[1] 黄丽萍[1] 吴萍萍[1] 陈晓兰[1] 

机构地区：[1]福建医科大学药学院,福州350004

出　　处：《药物分析杂志》2013年第4期566-571,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：福建省科技厅重点项目(2009I0009);福建省自然基金项目(2012J01368);福建医科大学教授基金项目(01601086)

摘　　要：目的:合成肌苷分子印迹聚合物并研究其吸附性能。方法:以肌苷为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了肌苷印迹聚合物(INO-MIP)。采用静态平衡吸附实验评价肌苷对底物分子的结合特性,并进行了吸附动力学研究和选择性实验。结果:INO-MIP对肌苷和次黄嘌呤呈现出了较高的选择吸附特性;Scatchard分析显示INO-MIP对肌苷存在两类结合位点,高亲和力结合位点的离解常数Kd=0.1757 mL.g-1,最大结合量Qmax=4.302μmol.g-1,低亲和力结合位点的离解常数Kd=1.6946 mL.g-1,最大结合量Qmax=22.957μmol.g-1。结论:合成的印迹聚合物对肌苷、次黄嘌呤具有较高的亲和力和特定的选择性,可望将其应用于肌苷和次黄嘌呤的体内药物分析中。Objective： To synthesize inosine molecularly imprinted polymer and study its adsorption properties.Methods：An inosine molecularly imprinted polymer（INO-MIP） was synthesized with inosine as template,acrylamide as functional monomer and ethylene glycol dimethacrylateas crosslinking agent.Inosine molecularly imprinted polymers＇ binding affinity and selectivity to inosine were evaluated by equilibrium adsorption affinity and its adsorption kinetics was observed.Results： INO-MIP exhibited good adsorption and selectivity toward inosine and hypoxanthine.Scatchard analysis revealed two classes of binding sites in INO-MIP with their dissociation constants（Kd） of 0.1757 mL·g^-1 and 1.6946 mL·g^-1,respectively,and the maximum binding capacity Qmax of 4.302 μmol·g^-1 and 22.957 μmol·g^-1,respectively.Conclusion： The synthesized molecularly imprinted polymer has high affinity and specific selectivity to inosine and hypoxanthine,which is expected to be applied in in vivo drug analysis.

关 键 词：分子印迹聚合物 肌苷 次黄嘌呤核苷 合成 吸附 Scatchard分析 体内药物分析 

分 类 号：R917[医药卫生—药物分析学]