羧基丁二酰β-环糊精微球电色谱柱的制备及手性分离  

Preparation and chiral separation of carboxyosuccinyl β-cyclodextrin microsphere electrochromatographic column

作　　者：李英杰[1] 丁莉[1] 吕仁江[1] 王立娜[1] 吴婧[1] 张福祥[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《药物分析杂志》2013年第4期572-575,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：黑龙江省自然科学基金项目(B201218)

摘　　要：目的:用羧基丁二酰β-环糊精微球固定相建立一种利用毛细管电色谱拆分手性对映体的方法。方法:以羧基丁二酰β-环糊精微球为手性固定相制备新型的毛细管电色谱填充柱。在毛细管电色谱模式下,对2种氨基酸和2种手性药物对映体进行拆分。结果:在优化色谱条件下,4种对映体均得到基线分离。结论:所制备的新型手性填充柱具有较强的手性拆分能力,且重现性好、操作简单。表明羧基丁二酰β-环糊精微球可作为电色谱柱的固定相用于电色谱手性分离。Objective：To establish a method of enantiomeric chiral separation by capillary electrochromatography（CEC） using carboxyosuccinyl β-cyclodextrin microspheres as the stationary phase.Methods：A novel CEC packed column was prepared using carboxyosuccinyl β-cyclodextrin microspheres as the chiral stationary phase.In capillary electrochromatographic mode,the prepared packed column was evaluated by separating racemic mixtures of two types of amino acids and two types of chiral drugs.Results：In the optimal CEC conditions,four enantiomers were baseline separated.Conclusion：It proved that the prepared chiral packed column has strong chiral separation ability,good reproducibility and is easy to operate.The results reveal that carboxyosuccinyl β-cyclodextrin microspheres can be applied as a CEC stationary phase for chiral electrochromatography.

关 键 词：羧基丁二酰β-环糊精微球 毛细管电色谱 固定相 填充柱 氨基酸 手性药物 对映体分离 

分 类 号：R917[医药卫生—药物分析学]