HPLC-ESI-MS分析郁舒颗粒的化学成分  

Identification and characterization of constituents in Yushu granules by HPLC-ESI-MS

作　　者：惠婷婷[1,2] 夏忠庭[2] 张兰兰[2] 吴廼峰[2] 陈晓鹏[2] 周水平[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津天士力集团研究院中药所,天津300410

出　　处：《药物分析杂志》2013年第4期586-594,共9页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立郁舒颗粒HPLC-ESI-MS分析方法,鉴定其化学成分。方法:高效液相色谱采用Agilent ZORBAXSB-C18色谱柱(4.6 mm×250 mm,5μm),以0.1%醋酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,检测波长为203 nm;质谱使用ESI离子源,正、负离子分别检测。结果:通过质谱分析以及参照相关文献共鉴定出46个成分,其中木脂素类成分12个、黄酮苷类成分8个、单萜苷类成分7个、皂苷类成分7个、有机酸类成分4个、间苯三酚类成分4个、鞣质类成分2个、糖酯类成分2个。结论:建立的方法灵敏度高、分离度好,为郁舒颗粒物质基础研究提供了一种快速准确的分析方法。Objective：To identify Yushu granules by using high performance liquid chromatography coupled with electrospray tandem mass spectrometry（HPLC-ESI-MS）.Methods：The analysis conditions were optimized as follows：Agilent ZORBAX SB-C18 column（4.6 mm×250 mm,5 μm）,mobile phase（A） 0.1% acetic acid phase（B） acetonitrile,gradient elution,detection wavelength at 203 nm.The analysis was performed in both negative and positive ion mode.Results： A total of 46 compounds were simultaneously identified by ESI/MS through comparison with reference standards and literature data,including 12 lignoids,8 flavonoid glycosides,7 monoterpene glycosides,4 organic acids,4 phloroglucinol derivatives,2 tannins and 2 sugur esters.Conclusion：The method is sensitive,and provides good and rapid qualitative characterization of the Yushu granules composition.

关 键 词：高效液相色谱法 液质联用 中成药成分鉴定 绿原酸 芍药内酯苷 芍药苷 芦丁 金丝桃苷 异槲皮苷 槲皮苷 槲皮素 五味子醇甲 戈米辛J 五味子酯甲 五味子甲素 五味子乙素 

分 类 号：R917[医药卫生—药物分析学]