钙化胎盘及脐带血中纳米细菌的形态学观察  

Morphological observation of nanobacteria in calcified placenta and umbilical cord blood

作　　者：刘胜男[1] 张德纯[1] 张名均[1] 郭亚楠[1] 杨晓容[1] 杨顺杰[2] 

机构地区：[1]重庆医科大学基础医学院病原生物学教研室,分子医学与肿瘤研究中心,重庆400016 [2]重庆医科大学生命科学院电镜组,重庆400016

出　　处：《临床检验杂志》2013年第3期215-217,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(31000075)

摘　　要：目的研究纳米细菌(nanobacteria,NB)的分布,寻求检测胎盘钙化NB感染的更好组织来源。方法收集孕妇胎盘钙化组织和脐带血20例,并以20例正常胎盘组织作对照,分离培养NB;用革兰染色和茜素红染色法、扫描电镜和透射电镜进行形态学鉴定。结果培养沉淀物符合NB的特性。脐带血NB分离率为80%(16/20),钙化胎盘组织为65%(13/20),均明显高于正常胎盘组织的10%(2/20),χ2分别为12.07、9.09,P均<0.01;脐带血与钙化胎盘组织比较,差异无统计学意义(χ2=1.33,P>0.05)。油镜下脐带血和钙化胎盘组织分离的NB为球状或球杆状,形态相似;茜素红染色脐带血中NB较多且聚集成簇;透射电镜和扫描电镜下NB呈球形,大小为80～500 nm,并可见毛刺结构。结论胎盘钙化与NB感染有关,脐带血适用于检测钙化胎盘的NB感染。Objective To study the distribution of nanobacteria（NB） and explore an appropriate tissue as the source for detection of placental calcification infected with nanobacteria.Methods The calcified placental tissues and umbilical cord blood from 20 pregnant women for isolation and culture of NB,and normal placental tissues were used as controls.The morphological identification of NB was performed with Gram and alizarin red staining and scanning electron microscope（SEM）,transmission electron microscope（TEM）.Results The precipitates were corresponding to the characteristics of NB.The isolation rate of NB was 65%（13/20） in the specimens of calcified placental tissues and 80%（16/20） in the cord blood,and both of them were higher than that in normal placental tissues（10%） with a significantly statistical difference（chi-square values were 12.07 and 9.09 respectively,P＆lt;0.01）,but there was no significant difference for the positive isolation rate of NB between the umbilical cord blood and calcified placental tissues（chi-square value was 1.33,P＆gt;0.05）.The shape of NB isolated from calcified placental tissue and umbilical cord blood under oil lens was spherical or rhabdoid with burr edge,and was globoid with size of 80-500 nm and staining of Gram-negative,Alizarin Red-positive under electron microscope.Conclusion Nanobacteria infection may be relevant with placental calcification.The specimens from umbilical cord blood should be more suitable for detecting NB in calcified placental tissues.

关 键 词：纳米细菌 胎盘钙化 脐带血 

分 类 号：R714.56[医药卫生—妇产科学] R372[医药卫生—临床医学]