黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞毒性研究  

Toxicity Study of the Uptaking Compositions of Diosooroa Bulbifera L Alcohol Extract on HL-7702 and HepG2 Cell

作　　者：陈莹蓉[1] 王翔[1] 闵丽姗[1] 戴利成[1] 杨水新[2] 

机构地区：[1]湖州市中心医院中心实验室湖州市分子医学重点实验室,浙江湖州313000 [2]湖州市中心医院临床药理科,浙江湖州313000

出　　处：《中国现代应用药学》2013年第4期368-372,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：2011年湖州市科技局一般科研计划项目(2011YS02)

摘　　要：目的研究黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞的毒性。方法 75%乙醇回流提取得黄药子提取物,高、中、低剂量作用于Caco-2细胞,以黄药子醇提物Caco-2细胞摄取液作用于HL-7702和HepG2细胞,进行细胞活性实验,测定生化指标ALT、AST、GSH-PX和MDA值。结果与对照组比,高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用后,HL-7702和HepG2细胞的存活率显著降低(P<0.01);高剂量组黄药子醇提物Caco-2细胞摄取液作用72 h后,HL-7702细胞上清液中ALT、AST显著升高(P<0.01);高剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后,HepG2细胞上清液中ALT、AST显著升高(P<0.01)。高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后,HL-7702和HepG2细胞上清液中MDA显著升高,GSH-PX显著降低(P<0.01)。结论黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞有毒性。OBJECTIVE To study the toxicity of the uptaking compositions of Diosooroa bulbifera L alcohol extract on HL-7702 and HepG2 cell. METHODS After reflux extraction with 75% alcohol, the alcohol extracts ofDiosooroa bulbifera L at high, middle and low doses were added to Caco-2 cell model. Then the uptaking compositions were added to HL-7702 and HepG2 cell for the determination of the cytoactive and the biochemical indicator of ALT, AST, GSH-PX and MDA. RESULTS Compared with the control group, the high and middle dose groups decreased the survival rate of HL-7702 and HepG2 cell （P〈0.01）. The high dose group increased ALT and AST of HL-7702 cell supernatant after 72 h （P〈0.01）. The high dose group increased ALT and AST of HepG2 cell supernatant after 48 h and 72 h （P〈0.01）. MDA were significant increased and GSH-PX were significant decreased in the supernatant of HL-7702 and HepG2 cell at high and middle doses after 48 h and 72 h （P〈0.01）. CONCLUSION The result showed that the uptaking compositions ofDiosooroa bulbifera L alcohol extract had toxic effect in HL-7702 and HepG2 cell.

关 键 词：黄药子 CACO-2细胞 肝细胞 毒性 

分 类 号：R991[医药卫生—毒理学]