宣痹通络汤对大鼠心肌缺血/再灌注损伤的保护作用  

Protective Effects of Xuanbi Tongluo Decoction On Myocardial Ischemia/reperfusion Injury In Rats

作　　者：潘力弢[1] 郑运英[1] 曹田梅[1] 

机构地区：[1]深圳市第二人民医院中医科,广东深圳518081

出　　处：《新中医》2013年第5期160-163,共4页New Chinese Medicine

基　　金：深圳市科技局立项课题(编号:201203038)

摘　　要：目的:探讨宣痹通络汤对大鼠心肌缺血/再灌注损伤的保护作用及其机制。方法:大鼠40只,随机分成4组,假手术组、模型组、消心痛组和宣痹通络汤组,宣痹通络汤组行心肌缺血再灌注处理前,每天每只大鼠灌胃宣痹通络汤2mL/100g,连续15天,每天1次,消心痛组灌胃等量消心痛溶液,其余各组灌胃等量生理盐水;然后除假手术组外其余3组结扎冠状动脉左前降支,局部缺血30min后行60min血液再灌注。实验结束后测定心肌梗死的范围;股静脉取血,测定血清中肌酸激酶(CK)和乳酸脱氢酶(LDH)含量,同时检测丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:与模型组比较,宣痹通络汤和消心痛均能缩小心肌缺血/再灌注损伤大鼠的心肌梗死范围,降低血清CK与LDH含量,提高心肌SOD活性、减少MDA含量,差异均有显著性意义(P<0.05);而与消心痛组比较,宣痹通络汤组的缩小心肌梗死范围、降低血清CK与LDH含量,提高心肌SOD活性、减少MDA含量作用更为明显,差异均有显著性意义(P<0.05)。结论:宣痹通络汤对大鼠心肌缺血/再灌注损伤具有保护作用,其抗脂质过氧化损伤、降低氧化应激,减少心肌细胞凋亡的发生,是其保护作用机制之一。Objective： To investigate the protection and mechanisms of Xuanbi Tongluo Decoction（XTD） in rats with my- ocardial ischemia/reperfusion injury（MI/RI）. Methods： Forty Wistar rats were randomly divided into four groups, sham opera- tion group, ischemia model group, isosorbide dinitrate group and XTD group. The rats in XTD group and isosorbide dinitrate group were daily given gastric gavage of XTD（2 mL/100g）, isosorbide dinitrate respectively before ischemia-reperfusion, and the treatment lasted 15 days. The other two groups were treated with the same amount of normal saline. Except the sham operation group, rats in the other three groups were subjected to 30 minutes of ischemia by occlusion of the left anterior de- scending branches and 60 minutes of reperfusion. After completion of the experiment, the infarct area were assessed after dyeing with Evans blue and triphenyltet razolium chloride. Blood samples were taken from the abdominal aorta, and serum creatine kinase（CK） and lactate dehydrogenase（LDH） levels as well as superoxide dismutase（SOD） and malondialdehyde（MDA） activities were detected. Results： Compared with the model group, infarct area and serum CK and LDH contents were sig- nificantly decreased（P 〈 0.05）, the activity of myocardial SOD was significantly increased（P 〈 0.05） and the myocardial MDA content was significantly decreased（P 〈 0.05） in isosorbide group and XTD group, the effect in XTD group being superior to isosorbide group（P 〈 0.05）. Conclusion： Xuanbi Tongluo Decoction has obviously protective effect on rat MI/RI.

关 键 词：宣痹通络汤 心肌缺血 再灌注损伤 大鼠 

分 类 号：R285.5[医药卫生—中药学]