骨生长肽羧基端5肽衍生物H13D联合阿仑膦酸钠对去卵巢模型大鼠骨代谢的影响  

Effects of Osteogenic Growth Peptide C-terminal Pentapeptide Derivatives H13D Combined with Alendronate on Bone Metabolism in Ovariectomized Rats

作　　者：陈玲[1] 蔡旺[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《中国药房》2013年第17期1563-1565,共3页China Pharmacy

摘　　要：目的:探讨骨生长肽羧基端5肽衍生物H13D联合阿仑膦酸钠(Alen)对去卵巢模型大鼠骨代谢的影响。方法:将大鼠随机分为假手术组、模型组、H13D(10nmol/100g)组、Alen(10μg/100g)组及其联用组,每组8只,皮下注射相应药物,12周后检测各组大鼠腰椎骨(L1～L4)和股骨的骨密度(BMD)、血清生化指标[Ca、P、碱性磷酸酶(ALP)、骨钙素(BGP)]、骨形态学变化。结果:各组大鼠血清中Ca、P水平比较均无明显变化。与假手术组比较,其余各组大鼠ALP、BGP水平均升高,仅模型组和联用组有统计学差异(P<0.05或P<0.01);与模型组比较,其余各组大鼠ALP、BGP水平均降低,仅H13D组和Alen组有统计学差异(P<0.05);骨密度和骨形态学比较表明,联用组优于假手术组(P<0.05),假手术组优于Alen组(P<0.05),Alen组优于H13D组,H13D组优于模型组(P<0.05)。结论:H13D联合Alen治疗可明显增加去卵巢模型大鼠骨密度,改善骨微结构,具有明显的协同作用。OBJECTIVE： To explore the effect of osteogenic growth peptide C-terminal pentapeptide derivatives H13D combined with alendronate （Alen） on bone metabolism in ovariectomized （OVX） rats. METHODS： SD rats were randomly divided into sham operation group, model group, H13D （10 nmol/100 g） group, Alen （10 μg/100 g） group and drug combination group with 8 rats in each group. They were given relevant medicine subcutaneously. 12 weeks later, BMD, serum biochemical indicator （Ca, P, ALP, BGP） and bone morphological changes of lumbar spinal segment （LI-L4） and femur were measured. RESULTS： The levels of Ca and P in serum of rats had no significant change. Compared with sham operation group, the levels of ALP and BGP increased in other groups, and there was statistical difference in model group and drug combination group （P〈0.05 or P〈 0.01） ; compared with model group, the levels of ALP and BGP decreased in other groups, and there was statistical difference in H13D group and Alen group （P〈0.05）. BMD and bone morphology showed drug combination group was better than sham operation group （P〈0.05） ; sham operation group was better than Alen group （P〈0.05） ; Alen group was better than H13D group; H13D group was better than model group （P〈0.05）. CONCLUSIONS： H13D combined with Alen can significantly increase BMD of OVX rats, improve the micro-structure of the bone, which have obvious synergies.

关 键 词：骨质疏松症 大鼠 骨生长肽羧基端5肽衍生物H13D 阿仑膦酸钠 骨密度 骨形态学 骨代谢 

分 类 号：R965[医药卫生—药理学]