衍生化后紫外分光光度法测定注射用氨磷汀的含量  

Content Determination of Amifostine for Injection by UV Spectrophotometry after Derivatization

作　　者：黄滔敏[1] 陈念祖[1] 王胜资[2] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院药剂科,上海200031 [2]复旦大学附属眼耳鼻喉科医院放疗科,上海200031

出　　处：《中国药房》2013年第17期1596-1597,共2页China Pharmacy

基　　金：上海市科学技术委员会医学引导类资助项目(No.09411962000);上海市浦东新区卫生局卫生科技资助项目(No.PW2009D-7)

摘　　要：目的:建立注射用氨磷汀的含量测定方法。方法:以乙酰丙酮-甲醛为衍生化试剂,硼酸-醋酸为缓冲液,将氨磷汀经衍生化后采用紫外分光光度法测定其含量,检测波长为369nm。结果:氨磷汀检测质量浓度线性范围为0.75～5mg/m(lr=0.9991),平均回收率为100.61%,RSD为1.94%(n=3)。结论:该方法简便、快速、准确、重复性好,适用于注射用氨磷汀的含量测定。OBJECTIVE： To develop a method for the content determination of Amifostine for injection. METHODS： After derivatization of amifostine, UV spectrophotometry was selected to determine the content of amifostine based on acetylacetone-foimaldchyde as derivatized reagent and boric acid-acetic acid as buffer. The detection wavelength was 369 nm. RESULTS： The linear range of amifostine was 0.75-5 mg/ml （r=0.999 1） with an average recovery of 100.61% （RSD=1.94% , n=3）. CONCLUSIONS： The method is proved to be simple, rapid, accurate and reproducible, and it is suitable for the content determination of Amifostine for injection.

关 键 词：注射用氨磷汀 紫外分光光度法 衍生化 含量测定 

分 类 号：R927.2[医药卫生—药学]